||RecombinantE.coli FUR was expressed in E.coli and purified by using conventional chromatography techniques, 16.7kDa (148aa).
||Fur (ferric uptake regulator) protein is a DNA-binding protein which regulates iron-responsive genes. Fur is a small, 17-kDa, global transcriptional repressor that in the presence of iron regulates functions as diverse as iron acquisition, oxidative stress, and virulence. In Escherichia coli, members of the Fur family regulate the expression of more than 100 genes that function in processes as varied as the biosynthesis and transport of siderophores, the expression of virulence factors, the alleviation of oxidative and NO-induced stress, and the inhibition of ferritin production through the expression of RyhB.
||Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 2 mM CaCl2, 100 mM NaCl
||> 95% by SDS - PAGE.
||1 mg/ml (determined by Bradford assay)
|Sequences of amino acids:
||MTDNNTALKK AGLKVTLPRL KILEVLQEPD NHHVSAEDLY KRLIDMGEEI GLATVYRVLN QFDDAGIVTR HNFEGGKSVF ELTQQHHHDH LICLDCGK VI EFSDDSIEAR QREIAAKHGI RLTNHSLYLY GHCAEGDCRE DEHAHEGK
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.