Recombinant Human Heat Shock Transcription Factor 1, His-tagged HSF1-327H

Recombinant Human Heat Shock Transcription Factor 1, His-tagged

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Recombinant Human Heat Shock Transcription Factor 1, His-tagged

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Cat. No. : HSF1-327H
Product Overview : Recombinant HSF1, fused to His tag, was expressed in E.coli and purified by using conventional chromatography techniques (549 aa, 59.4 kDa).
Description : Heat shock factor 1 (HSF1) is a major transactivator of heat shock proteins in response to environmental changes, and it is also involved in oogenesis, spermatogenesis, and placental development. Best known for its involvement in heat shock response, Hsf1p regulates the transcription of hundreds of targets, including genes involved in protein folding, detoxification, energy generation, carbohydrate metabolism, and cell wall organization.
Source : E.coli.
Formulation : Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM DTT, 50 mM NaCl.
Molecular Weight : 59.4 kDa (549aa).
Purity : > 75% by SDS – PAGE.
Concentration : 1 mg/ml (determined by Bradford assay).
Sequence : MGSSHHHHHH SSGLVPRGSH MDLPVGPGAA GPSNVPAFLT KLWTLVSDPD TDALICWSPS GNSFHVFDQG QFAKEVLPKY FKHNNMASFV RQLNMYGFRK VVHIEQGGLV KPERDDTEFQ HPCFLRGQEQ LLENIKRKVT SVSTLKSEDI KIRQDSVTKL LTDVQLMKGK QECMDSKLLA MKHENEALWR EVASLRQKHA QQQKVVNKLI QFLISLVQSN RILGVKRKIP LMLNDSGSAH SMPKYSRQFS LEHVHGSGPY SAPSPAYSSS SLYAPDAVAS SGPIISDITE LAPASPMASP GGSIDERPLS SSPLVRVKEE PPSPPQSPRV EEASPGRPSS VDTLLSPTAL IDSILRESEP APASVTALTD ARGHTDTEGR PPSPPPTSTP EKCLSVACLD KNELSDHLDA MDSNLDNLQT MLSSHGFSVD TSALLDLFSP SVTVPDMSLP DLDSSLASIQ ELLSPQEPPR PPEAENSSPD SGKQLVHYTA QPLFLLDPGS VDTGSNDLPV LFELGEGSYF SEGDGFAEDP TISLLTGSEP PKAKDPTVS
Storage : Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Gene Name : HSF1
Synonyms : HSF1; heat shock transcription factor 1; HSTF1; HSF 1.
Gene ID : 3297
mRNA Refseq : NM_005526.2
Protein Refseq : NP_005517.1
MIM : 140580
UniProt ID : Q00613
Chromosome Location : 8q24.3
Function : protein binding; sequence-specific DNA binding; transcription factor activity.

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