||Recombinant human Interleukin 32, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography,19.1 kDa (168aa).
||Interleukin 32 isoform A belongs to cytokine family. This protein expression is increased after the activation of T-cells by mitogens or the activation of NK cells by IL-2. It has the ability to induce proinflammatory cytokines such as TNFα and IL8 in THP1 cells, and activates typical cytokine signaling pathways involving NFκB and p38. IL-32 can also support osteoclast differentiation but not osteoclast activation by regulating the MAPK/ERK pathway and the actin cytoskeleton.
|Sequences of amino acids:
||MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMCF PKVLSDDMKK LKARMHQAIE RFYDKMQNAE SGRGQVMSSL AELEDDFKEG YLETVAAYYE EQHPELTPLL EKERDGLRCR GNRSPVPDVE DPATEEPGES FCDKSYGAPR GDKEELTPQK CSEPQSSK
||Liquid. In20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol
||> 95% by SDS - PAGE
||1 mg/ml (determined byBradford assay)
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.