||Recombinantextracellular domain of LAIR-1( 22-125aa) was overexpressed in E.coli, and purified by using conventional chromatography techniques (105 aa, 11.7 kDa).
||LAIR-1 is a glycoprotein(~32kDa) expressed on the surface of the majority of human peripheral blood mononuclear leukocytes including T cells , B cells, NK cells, macrophages and dendritic cells. LAIR-1 functions as an inhibitory receptor in NK cells, T cells and B cells. Lair-1 consists of a leader sequence, extracellular domain, transmembrane domain, cytoplasmic region.
||Liquid. In 20 mM Tris-HCl buffer (pH 7.5) containing 50 mM NaCl, 1 mM EDTA.
||11.7 kDa (105 aa).
||> 95% by SDS – PAGE.
||1 mg/ml (determined by Bradford assay).
||MQEEDLPRPS ISAEPGTVIP LGSHVTFVCR GPVGVQTFRL ERESRSTYND TEDVSQASPS ESEARFRIDS VSEGNAGPYR CIYYKPPKWS EQSDYLELLV KETSG
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.