Recombinant Human O-6-methylguanine-DNA Methyltransferase, His-tagged

• Cat.No.: MGMT-121H
• Product Overview: Recombinant MGMT, fused to His-tag at N-terminus, was expressed in E.coli and was purified by conventional chromatography techniques,23.8 kDa (227 aa), (Real molecular weight on SDS-PAGE will be shift up).

Specification

• Cat. No.: MGMT -121H
• Description: O-6-methylguanine-DNA methyltransferase (MGMT) is an enzyme that repairs O-6-methylguanine, a mutagenic DNA base damaged by endogenous and environmental alkylating agents and is involved in the cellular defense against the biological effects of O-6-methylguanine in DNA. MGMT repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. There are few reports that abnormal MGMT expression correlates with the prognosis in human solid cancers.
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MDKDCEMKRT TLDSPLGKLE LSGCEQGLHE IKLLGKGTSA ADAVEVPAPA AVLGGPEPLM QCTAWLNAYF QPEAIEEFP VPAFHHPVFQ QESFTRQVLW KLLKVVKFGE VISYQQLAAL AGNPKAARAV GGAMRGNPVP ILIPCHRVVC SSGAVGNYSG GLAVKEWLLA HEGHRLGKPG LGGSSGLAGA WLKGAGATSG SPPAGRN
• Formulation: Liquid. In 20 mM Tris-HCl buffer (pH 7.5) containing 1 mM DTT, 10% glycerol
• Purity: > 95% by SDS - PAGE
• Concentration: 0.5 mg/ml (determined by Bradford assay)
• Endotoxin Level: < 1.0 EU per 1 g of protein (determined by LAL method)
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Publication:
  Fluorogenic Real-Time Reporters of DNA Repair by MGMT, a Clinical Predictor of Antitumor Drug Response (2016)
  Activatable Probes for Anti-Cancer Therapy (2019)

Gene Information

• Gene Name: MGMT O-6-methylguanine-DNA methyltransferase [ Homo sapiens ]
• Synonyms: O-6-methylguanine-DNA-alkyltransferase; O-6-methylguanine-DNA methyltransferase; EC 2.1.1.63; 6-O-methylguanine-DNA methyltransferase; OTTHUMP00000020741; MGMT; O6-methylguanine-DNA methyltransferase; methylguanine-DNA methyltransferase
• Gene ID: 4255
• mRNA Refseq: NM_002412
• Protein Refseq: NP_002403
• MIM: 156569
• UniProt ID: P16455
• Chromosome Location: 10q26
• Pathway: DNA Repair
• Function: DNA binding; DNA-methyltransferase activity; metal ion binding; transferase activity ; methylated-DNA-[protein]-cysteine S-methyltransferase activity; zinc ion binding ; methylated-DNA-[protein]-cysteine S-methyltransferase activity.