"MGMT" Related Products

Expand
Expand
Expand

Recombinant Human O-6-methylguanine-DNA Methyltransferase, His-tagged

Cat. No. : MGMT -121H
Product Overview : Recombinant MGMT, fused to His-tag at N-terminus, was expressed in E.coli and was purified by conventional chromatography techniques,23.8 kDa (227 aa), (Real molecular weight on SDS-PAGE will be shift up).
Description : O-6-methylguanine-DNA methyltransferase (MGMT) is an enzyme that repairs O-6-methylguanine, a mutagenic DNA base damaged by endogenous and environmental alkylating agents and is involved in the cellular defense against the biological effects of O-6-methylguanine in DNA. MGMT repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. There are few reports that abnormal MGMT expression correlates with the prognosis in human solid cancers.
Sequences of amino acids : MGSSHHHHHH SSGLVPRGSH MDKDCEMKRT TLDSPLGKLE LSGCEQGLHE IKLLGKGTSA ADAVEVPAPA AVLGGPEPLM QCTAWLNAYF QPEAIEEFP VPAFHHPVFQ QESFTRQVLW KLLKVVKFGE VISYQQLAAL AGNPKAARAV GGAMRGNPVP ILIPCHRVVC SSGAVGNYSG GLAVKEWLLA HEGHRLGKPG LGGSSGLAGA WLKGAGATSG SPPAGRN
Formulation : Liquid. In 20 mM Tris-HCl buffer (pH 7.5) containing 1 mM DTT, 10% glycerol
Purity : > 95% by SDS - PAGE
Concentration : 0.5 mg/ml (determined by Bradford assay)
Endotoxin Level : < 1.0 EU per 1 g of protein (determined by LAL method)
Storage : Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Publication :
Gene Name : MGMT O-6-methylguanine-DNA methyltransferase [ Homo sapiens ]
Synonyms : O-6-methylguanine-DNA-alkyltransferase; O-6-methylguanine-DNA methyltransferase; EC 2.1.1.63; 6-O-methylguanine-DNA methyltransferase; OTTHUMP00000020741; MGMT; O6-methylguanine-DNA methyltransferase; methylguanine-DNA methyltransferase
Gene ID : 4255
mRNA Refseq : NM_002412
Protein Refseq : NP_002403
MIM : 156569
UniProt ID : P16455
Chromosome Location : 10q26
Pathway : DNA Repair
Function : DNA binding; DNA-methyltransferase activity; metal ion binding; transferase activity ; methylated-DNA-[protein]-cysteine S-methyltransferase activity; zinc ion binding ; methylated-DNA-[protein]-cysteine S-methyltransferase activity.

Online Inquiry

Note: There will be extra charge for optional service!

Please input "biomart" as verification code. Please review Creative BioMart's privacy policy for more information

Optional requirements on this protein    +Expand

Price Inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.