||Recombinant human IPP-1, fused to His-tag at C-terminus, was expressed in E.coli and purified by using conventional chromatography techniques (179 aa, 20 kDa).
||Protein phosphatase inhibitor-1(IPP-1) plays an important role in the regulation of glycogen metabolism through inhibition of type-1 protein serine/threonine phosphatase(PP1) activity, and it has been implicated in the regulation of cell growth. IPP-1 activation may impose cAMP control over proteins that are not directly phosphorylated by PKA. In the presence of calcium, PPI-1 is inactivated by calcineurin (or PP2B).
||Liquid. In 50 mM Tris-HCl buffer (pH 8.0) containing 1 mM DTT, 1 mM EDTA, 0.1 mM PMSF, 10% glycerol.
||20 kDa (179 aa).
||> 90% by SDS – PAGE.
||1 mg/ml (determined by Bradford assay).
||MEQDNSPRKI QFTVPLLEPH LDPEAAEQIR RRRPTPATLV LTSDQSSPEI DEDRIPNPHL KSTLAMSPRQ RKKMTRITPT MKELQMMVEH HLGQQQQGEE PEGAAESTGT QESRPPGIPD TEVESRLGTS GTAKKTAECI PKTHERGSKE PSTKEPSTHI PPLDSKGANS VLEHHHHHH
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.