Active Native Porcine pancreatic elastase

• Cat.No.: CELA1-52P
• Product Overview: Chromatographically prepared from the euglobin fraction of porcine pancreas by the method of Lewis et al. then twice crystallized. Supplied as a salt free lyophilized powder that is free of trypsin and chymotrypsin. 95percent pure by SDS-PAGE.

Specification

• Source: Porcine pancreas
• Species: Porcine
• Form: Lyophilized powder
• Bio-activity: 135 U/mg on substrate elastin-orcein (570 nm)
• Molecular Mass: 25,500
• Purity: >95% by SDS-PAGE analysis
• Usage: For in vitro laboratory use only
• Notes: Optimal experimental conditions and working dilutions must be determined by the end user. Sample concentration and volume are for example purposes only and do not necessarily represent the product that will be received. Please contact us for a current lot-specific product datasheet.
• Storage: 4°C
• Storage Buffer: Salt free
• Reconstitution: Reconstitute with 0.05M Na Acetate 0.1M NaCl pH 5.0 to 1 mg/ml or desired concentration, aliquot and freeze remaining portion. Assay: 5-10 ug in 0.1M Tris pH 8.8 at 37 C containing 0.2mM Suc-(Al)3-pNA.
• Shipping: Cold pack

Gene Information

• Gene Name: CELA1 chymotrypsin-like elastase family, member 1 [ Sus scrofa ]
• Official Symbol: CELA1
• Synonyms: ELA1; ELS1; Elastase-1; chymotrypsin-like elastase family member 1; pancreatic elastase I
• Gene ID: 396766
• mRNA Refseq: NP_998988
• Protein Refseq: NM_213823
• UniProt ID: P00772
• Chromosome Location: 5; 5
• Function: metal ion binding; protein binding; serine-type endopeptidase activity