Recombinant Human Prolactin Receptor, chiMAX Fc Fusion Protein

• Cat.No.: PRLR-76H
• Product Overview: Prolactin is a neuroendocrine peptide hormone that is secreted primarily by the pituitary gland. Prolactin receptor is a class 1 cytokine transmembrane receptor that varies in size with tissue source and species. It consists of an extracellular region with 5 cysteines which contains the prolactin binding site, a single transmembrane domain and a cytoplasmic region. The extracellular domain (Gln25-Asp234) is expressed recombinantly, purified by chromatography, sterile filtered and lyophilized. >95percent pure by SDS-PAGE and biologically active as measured by inhibitory activity toward prolactin-induced proliferation of Nb2-11 rat lymphoma cells (Gout et al. (1980) Cancer Res. 40:2433). ED50 for this effect is typically< 1.0="" ug/ml="" in="" the="" presence="" of="" 0.5="" ng/ml="" of="" recombinant="" human="" prolactin.="" add="" deionized="" water="" to="" original="" volume,="" aliquot="" and="" freeze="" unused="">This chiMAX Fc Fusion Protein is glycosylated and expressed as a chimera with the Fc region of mouse immunoglobulin (heavy chain hinge, CH2 and CH3). Fc fusion proteins are typically more stable and resistant to degradation and clearance than native cytokines. Fc fusion proteins appear as a dimer in SDS-PAGE under non-reducing conditions.

Specification

• Source: Insect cell
• Species: Human
• Form: Lyophilized powder
• Molecular Mass: 51,000
• Purity: >95% by SDS-PAGE analysis
• Characteristic: ED50< 1 ug/ml in the presence of 0.5 ng/ml of recombinant human prolactin as determined in a cell proliferation assay using nb2-11 rat lymphoma>
• Usage: For in vitro laboratory use only
• Notes: Optimal experimental conditions and working dilutions must be determined by the end user. Sample concentration and volume are for example purposes only and do not necessarily represent the product that will be received. Please contact us for a current lot-specific product datasheet.
• Storage: -70°C
• Storage Buffer: PBS
• Shipping: Cold pack

Gene Information

• Gene Name: PRLR prolactin receptor [ Homo sapiens ]
• Official Symbol: PRLR
• Synonyms: PRLR; prolactin receptor; PRL-R; hPRL receptor; secreted prolactin binding protein; hPRLrI
• Gene ID: 5618
• mRNA Refseq: NM_000949
• Protein Refseq: NP_000940
• MIM: 176761
• UniProt ID: P16471
• Chromosome Location: 5p14-p13
• Pathway: Adipogenesis, organism-specific biosystem; Cytokine Signaling in Immune system, organism-specific biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; ErbB4 signaling events, organism-specific biosystem; Growth hormone receptor signaling, organism-specific biosystem; Immune System, organism-specific biosystem
• Function: cytokine receptor activity; metal ion binding; ornithine decarboxylase activator activity; peptide hormone binding; prolactin receptor activity; protein homodimerization activity; receptor activity