Recombinant Human TNF Protein, FLAG-tagged
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|Product Overview :||Recombinant Human TNF-alpha with FLAG tag was expressed in E.coli.|
|Description :||This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.|
|Source :||E. coli|
|Bio-activity :||Induces cell death at a concentration range of 0.01-10 ng/ml (ED50= 0.05 ng/ml or 2 x 10E7 Units/mg; WEHI 164 cells).|
|Molecular Mass :||~17kDa|
|Endotoxin :||<0.01 EU per 1 μg of the protein by the LAL method|
|Purity :||>95% by SDS-PAGE|
|Storage :||Store at -20 to -80 centigrade in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.|
|Storage Buffer :||PBS|
|Reconstitution :||10 µg size: Reconstitute with 100 µl sterile water.
50 µg size: Reconstitute with 50 µl sterile water.
PBS containing at least 0.1% BSA should be used for further dilutions.
|Gene Name :||TNF tumor necrosis factor [ Homo sapiens (human) ]|
|Official Symbol :||TNF|
|Synonyms :||TNF; tumor necrosis factor; DIF; TNFA; TNFSF2; TNLG1F; TNF-alpha; tumor necrosis factor; APC1 protein; TNF, macrophage-derived; TNF, monocyte-derived; TNF-a; cachectin; tumor necrosis factor ligand 1F; tumor necrosis factor ligand superfamily member 2; tumor necrosis factor-alpha; tumor necrosis factor; APC1 protein; TNF, macrophage-derived; TNF, monocyte-derived; TNF-a; cachectin; tumor necrosis factor ligand 1F; tumor necrosis factor ligand superfamily member 2; tumor necrosis factor-alpha|
|Gene ID :||7124|
|mRNA Refseq :||NM_000594|
|Protein Refseq :||NP_000585|
|UniProt ID :||P01375|
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