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Active Recombinant Human Agouti Related Protein

Cat.No. : AGRP -63H
Product Overview : A DNA sequence encoding the amino acids Met 1 - Thr 132 of the human AgRP, the protein product of the Agouti-Related Transcript (ART) (Shutter, J.R. et al., 1997, Genes Dev. 11(5):593 - 602) was fused with a 6X histidine tag at the C-terminus. The protein was expressed in the insect cell line Sf21.
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Description : Agouti-Related Protein (AgRP), the protein product of the Agouti-Related Transcript (ART), is a neuropeptide that regulates energy metabolism and the development of obesity by antagonizing α-melanocyte stimulating hormone (α-MSH) on MC-3 and MC-4 receptors (1 - 4). AgRP is predominantly expressed in the hypothalamus and adrenal medulla (5). Mature human AgRP is a 112 aa peptide; its C-terminal portion contains ten conserved cysteines that form five disulfide bonds (5, 6). Human AgRP shares 82% and 72% aa sequence identity with mouse and rat AgRP, respectively. It shares 32% aa sequence identity with Agouti. As in the case of Agouti, the C-terminal cysteine-rich region is sufficient for biological activity (7). AgRP is 100 times more potent than Agouti in antagonizing MC-3 and MC-4 receptors (8). AgRP also induces induces the β-arrestin dependent endocytosis of MC-3 and MC-4 (9). Hypothalamic expression of AgRP is upregulated in obesity and diabetes (5, 10), and chronic AgRP administration increases food intake and weight gain in rats (11). Genetically-linked polymorphisms of AgRP in humans are associated with susceptibility to anorexia nervosa (12, 13). In addition, AgRP inhibits the ACTH-induced synthesis of steroid hormones in a mechanism that does not involve melanocortin receptors (14).References:1. Martin, N.M. et al., 2006, Peptides 27:333.2. Fan, W. et al., 1997, Nature 385:165.3. Ollmann, M.M. et al., 1997, Science 278:135.4. Arora, S. and Anubhuti, 2006, Neuropeptides 40:375.5. Shutter, J.R. et al., 1997, Genes Develop. 11:593.6. Kiefer, L.L. et al., 1998, Biochemistry 37:991.7. Jackson, P.J. et al., 2002, Biochemistry 41:7565.8. Fong, T.M. et al., 1997, Biochem. Biophys. Res. Commun. 237:629.9. Breit, A. et al., 2006, J. Biol. Chem. 281:37447.10. Katsuki, A. et al., 2001, J. Clin. Endocrinol. Metab. 86:1921.11. Small, C.K. et al., 2001, Diabetes 50:248.12. Vink, T. et al., 2001, Mol. Psychiatry 6:325.13. Dardennes, R.M. et al., 2007, Psychoneuroendocrinology 32:106.14. Doghman, M. et al., 2007, Mol. Cell. Endocrinol. 265 - 266:108.Note: This protein is designed for use as an ELISA control.
Source : Insect Sf21 cell line
Species : Human
Tag : His
Bio-activity : Bioactivity data is not available at this time. This preparation is intended for use as a control in AgRP ELISA.
Molecular Mass : Based on N-terminal sequencing, the mature recombinant human AgRP preparation contains peptides that start at Ala 21 and Leu 47. The recombinant human AgRP peptide have migrated with apparent molecular mass of approximately 14 - 19 kDa in SDS-PAGE under r
Endotoxin : < 1.0 eu per 1 μg of the cytokine as determined by the lal
Purity : >90%, as determined by SDS-PAGE and visualized by silver stain.
Storage : Upon reconstitution, this cytokine, in the presence of a carrier protein, can be stored under sterile conditions at 2 - 8° C for one month or at -20° C to -70° C in a manual defrost freezer for three months without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile PBS containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of no less than 10 μg/Ml.
Gene Name : AGRP agouti related protein homolog (mouse) [ Homo sapiens ]
Official Symbol : AGRP
Synonyms : AGRP; agouti related protein homolog (mouse); agouti (mouse) related protein; agouti-related protein; Agrt; ART; ASIP2; AGRT;
Gene ID : 181
mRNA Refseq : NM_001138
Protein Refseq : NP_001129
MIM : 602311
UniProt ID : O00253
Chromosome Location : 16q22
Pathway : Adipocytokine signaling pathway, organism-specific biosystem; Adipocytokine signaling pathway, conserved biosystem; Syndecan-3-mediated signaling events, organism-specific biosystem;
Function : neuropeptide hormone activity; receptor binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (10)

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Can AGRP be targeted for the treatment of obesity? 03/30/2022

AGRP and the melanocortin system are potential targets for developing anti-obesity therapies, and research is ongoing to develop selective agonists or antagonists to modulate AGRP signaling.

Is AGRP involved in other physiological processes besides appetite regulation? 03/04/2022

AGRP is also implicated in the modulation of autonomic functions, such as blood pressure regulation and cardiovascular control.

What are the downstream signaling pathways activated by AGRP? 03/02/2020

AGRP inhibits the signaling of melanocortin receptors, leading to reduced cAMP production and affecting intracellular pathways involved in appetite and energy regulation.

How is AGRP involved in the central regulation of energy balance? 02/24/2020

AGRP acts as a key neuropeptide in the hypothalamus, integrating signals from peripheral tissues to regulate energy homeostasis, appetite, and body weight.

What is the relationship between AGRP and the melanocortin system? 11/03/2019

AGRP functions as an antagonist to melanocortin receptors, counteracting the effects of α-melanocyte-stimulating hormone (α-MSH), which promotes satiety and energy expenditure.

Can AGRP interact with other neuropeptides in appetite regulation? 10/10/2019

Yes, AGRP can interact with other neuropeptides, such as neuropeptide Y (NPY), and together they influence the central regulation of appetite and energy balance.

Can alterations in AGRP expression contribute to eating disorders? 07/06/2019

Dysregulation of AGRP expression or activity has been suggested to contribute to the development of certain eating disorders, such as binge eating disorder or hyperphagia.

Can AGRP be used as a diagnostic marker for metabolic disorders? 05/27/2019

While AGRP itself is not typically used as a diagnostic marker, studying its expression levels or genetic variations may provide insights into the risk and development of metabolic disorders.

How does AGRP regulate appetite? 07/23/2018

AGRP acts on the melanocortin receptors in the brain, inhibiting their activation and resulting in an increase in appetite and a decrease in energy expenditure.

Can AGRP be used as a biomarker for monitoring the efficacy of weight loss interventions? 05/11/2018

AGRP levels may have potential as a biomarker for monitoring the response to weight loss interventions, but further research is needed to establish its clinical utility.

Customer Reviews (5)

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Reviews
09/21/2019

    This company offers a wide range of recombinant proteins at competitive prices.

    08/08/2019

      The pricing structure for their proteins is transparent and reasonable.

      07/04/2019

        The customer support team was extremely helpful and responsive, providing detailed information about the products.

        05/09/2018

          We are exceedingly satisfied with the quality of this protein.

          03/14/2018

            The exceptional quality of the proteins makes it a great value for the cost.

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