Native Human MMP-9/Lipocalin/TIMP-1 Complex

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Native Human MMP-9/Lipocalin/TIMP-1 Complex

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Cat.No. : MMP9-40H
Product Overview : Matrix Metalloproteinase 9–Lipocalin–TIMP-1 complex, Gelatinase B, 92-kDa Type IV Collagenase, EC 3.4.24.35). Prepared from human blood certified to be negative for HBsAg and for antibodies to HIV and HCV.
Source : Human neutrophil granulocytes (Buffy Coat)
Species : Human
Form : Liquid, in 50 mM Tris/HCl, pH 7.0, 200 mM NaCl, 5 mM CaCl2, 1 μM ZnCl2, 0.05% NaN3, 0.05% Brij 35.
Purity : >95%, homogenous by electrophoresis and Western Blotting analysis
Unit Definition : The specific activity can be assayed with the synthetic substrate N-(2,4)-dinitrophenyl-Pro-Gln-Gly-Ile-Ala-Gly-Gln-D-Arg (Dnp-petide) (Masui et al. 1977). One unit MMP catalyzes the hydrolysis of 1 μmol Dnp peptide/min at 37°C and pH 7.0.
Storage : The enzyme is very stable if stored at -80°C. Repeated freezing and thawing must be avoided.
Gene Name : MMP9 matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase) [ Homo sapiens ]
Official Symbol : MMP9
Synonyms : MMP9; matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase); CLG4B, matrix metalloproteinase 9 (gelatinase B, 92kD gelatinase, 92kD type IV collagenase) , matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase); matrix metalloproteinase-9; 92 kDa gelatinase; type V collagenase; macrophage gelatinase; 92 kDa type IV collagenase; matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase); GELB; CLG4B; MMP-9; MANDP2;
Gene ID : 4318
mRNA Refseq : NM_004994
Protein Refseq : NP_004985
MIM : 120361
UniProt ID : P14780
Chromosome Location : 20q12-q13
Pathway : Activation of Matrix Metalloproteinases, organism-specific biosystem; Bladder cancer, organism-specific biosystem; Bladder cancer, conserved biosystem; CXCR4-mediated signaling events, organism-specific biosystem; Degradation of the extracellular matrix, organism-specific biosystem; Endochondral Ossification, organism-specific biosystem; Extracellular matrix organization, organism-specific biosystem;
Function : collagen binding; fibronectin binding; metal ion binding; metalloendopeptidase activity; peptidase activity; protein binding; protein complex binding; zinc ion binding;

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