|Product Overview :
||Recombinant full-length human MMP-13 (EC 3.4.24.--) expressed in Spodoptera frugiperda (471 amino acid residues [1-mhpgvlaaf ------ rvmpansilw c-471], molecular weight 53820 Da). Swiss Prot P45452. This product is APMA activated, which had been removed after activation employing SEC and dialysis. The actMMP-13 shows predominately the app. 48-kDa form and faint the 50-kDa and app. 30-kDa form.
||50 mM Tris pH 6.5, 250 mM NaCl, 5 mM CaCl2, 1 μM ZnCl2
||>200 munits/mg (international unit 1 μmole/min/mg)
||>95%, homogenous by SDS-PAGE
||The enzyme can be kept at -80°C for many month. Repeated freezing and thawing must be avoided. Dilute and activate only the amount of enzyme you need, do not store enzyme in reaction buffer. The active 48 kDa enzyme undergoes very fast changes into a 30 kDa form which still retains enzymatic characteristics of the 48 kDa enzyme.