| Description : |
Neurofascin 155 (NF155) is a type I transmembrane glycoprotein that belongs to the L1CAM family of cell adhesion proteins.1,2 The rat NF155 cDNA encodes a 1240 amino acid (aa) precursor that contains a 24 aa signal sequence, a 1086 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 109 aa cytoplasmic domain. The ECD consists of six Ig-like domains and four fibronectin type III repeats, the second of which has an RGD motif. A splice variant of Neurofascin, NF186, lacks the RGD-containing fibronectin type III domain but instead has a mucin-like domain and an additional non-RGD fibronectin type III domain.3 Within shared regions of the ECD, rat NF155 shares 45% and 39% aa sequence identity with rat Nr-CAM and L1CAM, respectively, and 98% aa sequence identity with human and mouse NF155. NF155 is transiently expressed by oligodendr centigradeytes at the onset of axon myelination, whereas NF186 is neuronally expressed in nodes of Ranvier. |
| Source : |
Mammalian cells |
| Species : |
Rat |
| Tag : |
His |
| Form : |
Lyophilized from a 0.2 μm filtered solution in PBS. |
| Bio-activity : |
Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Optimal neurite outgrowth was observed when neurons were plated on 96 well culture plates that had been precoated with 50 μL/well of the rrNeurofascin solution at 1015 μg/mL. |
| Molecular Mass : |
Predicted Molecular Mass: 114.7 kDa.SDS PAGE: 135140 kDa, reducing conditions. |
| Endotoxin : |
<1.0 eu per 1 μg of the protein by the lal</1.0 |
| Purity : |
>97%, by SDSPAGE under reducing conditions and visualized by silver stain. |
| Usage : |
FOR RESEARCH USE ONLY |
| Quality Control Test : |
12 months from date of receipt, 20 to 70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, 20 to 70 centigrade under sterile conditions after reconstitution. |
| Reconstitution : |
Reconstitute at 100 μg/mL in sterile PBS. |
| Warning : |
Avoid repeated freeze-thaw cycles. |
