Active Native Human TGF- β1

• Cat.No.: TGFB1-21H
• Product Overview: Purified from human platelets. Human blood products should always be treated in accordance with universal handling precautions.

Specification

• Description: Transforming growth factor betas (TGF Betas) mediate many cell-cell interactions that centigradecur during embryonic development. Three TGF Betas have been identified in mammals. TGF Beta 1, TGF Beta 2 and TGF Beta 3 are each synthesized as precursor proteins that are very similar in that each is cleaved to yield a 112 amino acid polypeptide that remains ass centigradeiated with the latent portion of the molecule.
• Source: human platelets
• Species: Human
• Form: Lyophilized from a 0.2 μm filtered solution in 35% acetonitrile and 0.1% TFA, containing 50 μg of bovine serum albumin per 1 μg of cytokine.
• Bio-activity: Measured by its ability to inhibit the IL-4-dependent proliferation of mouse HT-2 cells. Resazurin dye was used in the assay as a fluorescence indicator for cell proliferation (Tsang, M. et al., 1995, Cytokine 7:389). The ED50 for this effect is typically 0.04 - 0.1 ng/mL.
• Molecular Mass: The mature disulfide-linked homodimeric human TGF-β1 contains two 112 amino acid residue peptides and has a predicted molecular mass of approximately 25 kDa.
• Endotoxin: < 1.0 eu per 1 μg of the cytokine as determined by the lal
• Purity: >97%, as determined by SDS-PAGE and visualized by silver stain.
• Usage: FOR RESEARCH USE ONLY
• Quality Control Test: Lyophilized samples are stable for up to twelve months from date of receipt at -20 centigrade to -70 centigrade. Upon reconstitution, this cytokine can be stored under sterile conditions at 2° - 8 centigrade for one month or at -20 centigrade to -70 centigrade in a manual defrost freezer for three months without detectable loss of activity. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile 4 mM HCl containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a st centigradek solution of no less than 1 μg/mL.
• Warning: Avoid repeated freeze-thaw cycles.

Gene Information

• Gene Name: TGFB1 transforming growth factor, beta 1 [ Homo sapiens ]
• Official Symbol: TGFB1
• Synonyms: TGFB1; transforming growth factor, beta 1; DPD1, TGFB; transforming growth factor beta-1; Camurati Engelmann disease; CED; TGFbeta; TGF-beta-1; TGF-beta 1 protein; latency-associated peptide; LAP; DPD1; TGFB
• Gene ID: 7040
• mRNA Refseq: NM_000660
• Protein Refseq: NP_000651
• MIM: 190180
• UniProt ID: P01137
• Chromosome Location: 19q13.1
• Pathway: ACE Inhibitor Pathway, organism-specific biosystem; ALK1 signaling events, organism-specific biosystem; Adipogenesis, organism-specific biosystem; Amoebiasis, organism-specific biosystem; Amoebiasis, conserved biosystem; Cell cycle, organism-specific biosystem; Cell cycle, organism-specific biosystem
• Function: enzyme binding; eukaryotic cell surface binding; growth factor activity; protein N-terminus binding; protein binding; protein heterodimerization activity; protein homodimerization activity; type II transforming growth factor beta receptor binding; type II transforming growth factor beta receptor binding