Recombinant Human PYGL, His-tagged

• Cat.No.: PYGL-43H
• Product Overview: Recombinant human PYGL, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Description: PYGL also known as Glycogen phosphorylase, switches from inactive phosphorylase B to active phosphorylase A by phosphorylation of serine residue 15. Activity of this enzyme is further regulated by multiple allosteric effectors and hormonal controls. The liver isozyme serves the glycemic demands of the body in general while the brain and muscle isozymes supply just those tissues.
• Source: E.coli
• Species: Human
• Tag: His
• Form: Liquid. In phosphate buffered saline (pH7.4) containing 30% glycerol, 1mM DTT.
• Molecular Mass: 100.7kDa (879aa)
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSEFELRRQ ASMAKPLTDQ EKRRQISIRG IVGVENVAEL KKSFNRHLHF TLVKDRNVAT TRDYYFALAH TVRDHLVGRW IRTQQHYYDK CPKRVYYLSL EFYMGRTLQN TMINLGLQNA CDEAIYQLGL DIEELEEIEE DAGLGNGGLG RLAACFLDSM ATLGLAAYGY GIRYEYGIFN QKIRDGWQVE EADDWLRYGN PWEKSRPEFM LPVHFYGKVE HTNTGTKWID TQVVLALPYD TPVPGYMNNT VNTMRLWSAR APNDFNLRDF NVGDYIQAVL DRNLAENISR VLYPNDNFFE GKELRLKQEY FVVAATLQDI IRRFKASKFG STRGAGTVFD AFPDQVAIQL NDTHPALAIP ELMRIFVDIE KLPWSKAWEL TQKTFAYTNH TVLPEALERW PVDLVEKLLP RHLEIIYEIN QKHLDRIVAL FPKDVDRLRR MSLIEEEGSK RINMAHLCIV GSHAVNGVAK IHSDIVKTKV FKDFSELEPD KFQNKTNGIT PRRWLLLCNP GLAELIAEKI GEDYVKDLSQ LTKLHSFLGD DVFLRELAKV KQENKLKFSQ FLETEYKVKI NPSSMFDVQV KRIHEYKRQL LNCLHVITMY NRIKKDPKKL FVPRTVIIGG KAAPGYHMAK MIIKLITSVA DVVNNDPMVG SKLKVIFLEN YRVSLAEKVI PATDLSEQIS TAGTEASGTG NMKFMLNGAL TIGTMDGANV EMAEEAGEEN LFIFGMRIDD VAALDKKGYE AKEYYEALPE LKLVIDQIDN GFFSPKQPDL FKDIINMLFY HDRFKVFADY EAYVKCQDKV SQLYMNPKAW NTMVLKNIAA SGKFSSDRTI KEYAQNIWNV EPSDLKISLS NESNKVNGN
• Purity: >80% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: PYGL phosphorylase, glycogen, liver [ Homo sapiens ]
• Official Symbol: PYGL
• Synonyms: PYGL; phosphorylase, glycogen, liver; phosphorylase, glycogen; liver; glycogen phosphorylase, liver form; glycogen storage disease type VI; Hers disease; GSD6
• Gene ID: 5836
• mRNA Refseq: NM_001163940
• Protein Refseq: NP_001157412
• MIM: 613741
• UniProt ID: P06737
• Chromosome Location: 14q11.2-q24.3
• Pathway: Glucose metabolism, organism-specific biosystem; Glycogen Metabolism, organism-specific biosystem; Glycogen breakdown (glycogenolysis), organism-specific biosystem; Insulin signaling pathway, organism-specific biosystem; Insulin signaling pathway, conserved biosystem; Metabolism, organism-specific biosystem; Metabolism of carbohydrates, organism-specific biosystem
• Function: AMP binding; ATP binding; bile acid binding; drug binding; glucose binding; glycogen phosphorylase activity; glycogen phosphorylase activity; nucleotide binding; protein homodimerization activity; purine base binding; pyridoxal phosphate binding; sugar binding; transferase activity, transferring glycosyl groups; vitamin binding