Recombinant Human PYCR2, His-tagged

• Cat.No.: PYCR2-46H
• Product Overview: Recombinant human PYCR2, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Description: PYCR2 also known as pyrroline-5-carboxylate reductase 2 isoform 1, belongs to the pyrroline-5-carboxylate reductase family. This protein catalyzes the conversion of pyrroline-5-carboxylate to proline, which is the last step in proline biosynthesis. The 3 substrates of this enzyme are L-proline, NAD+, and NADP+, whereas its 4 products are 1-pyrroline-5-carboxylate, NADH, NADPH, and H+.
• Source: E.coli
• Species: Human
• Tag: His
• Form: Liquid. In phosphate buffered saline (pH7.4) containing 50% glycerol, 5mM DTT, 1mM EDTA.
• Molecular Mass: 36kDa (343aa) confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMSVGFIG AGQLAYALAR GFTAAGILSA HKIIASSPEM NLPTVSALRK MGVNLTRSNK ETVKHSDVLF LAVKPHIIPF ILDEIGADVQ ARHIVVSCAA GVTISSVEKK LMAFQPAPKV IRCMTNTPVV VQEGATVYAT GTHALVEDGQ LLEQLMSSVG FCTEVEEDLI DAVTGLSGSG PAYAFMALDA LADGGVKMGL PRRLAIQLGA QALLGAAKML LDSEQHPCQL KDNVCSPGGA TIHALHFLES GGFRSLLINA VEASCIRTRE LQSMADQEKI SPAALKKTLL DRVKLESPTV STLTPSSPGK LLTRSLALGG KKD
• Purity: >90% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: PYCR2 pyrroline-5-carboxylate reductase family, member 2 [ Homo sapiens ]
• Official Symbol: PYCR2
• Synonyms: PYCR2; pyrroline-5-carboxylate reductase family, member 2; pyrroline-5-carboxylate reductase 2; P5CR2; P5CR 2; P5C reductase 2; pyrroline 5-carboxylate reductase isoform; FLJ54750
• Gene ID: 29920
• mRNA Refseq: NM_013328
• Protein Refseq: NP_037460
• UniProt ID: Q96C36
• Chromosome Location: 1q42.13
• Pathway: Arginine and proline metabolism, organism-specific biosystem; Arginine and proline metabolism, conserved biosystem; Metabolic pathways, organism-specific biosystem; Proline biosynthesis, glutamate =>proline, organism-specific biosystem; Proline biosynthesis, glutamate => proline, conserved biosystem
• Function: nucleotide binding; oxidoreductase activity; oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor; pyrroline-5-carboxylate reductase activity