Recombinant Human MSLN Protein, His-tagged

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Recombinant Human MSLN Protein, His-tagged

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Cat.No. : MSLN-001H
Product Overview : The recombinant human MSLN is expressed as a 317-amino acid protein consisting of Glu296 - Ser592 region of MSLN (UniProt accession #Q13421, isoform 2) and a C-terminal His-tag. It contains 4 potential N-linked glycosylation sites.
Source : HEK293
Species : Human
Tag : His
Bio-activity : Binds to CA125/MUC16 and anti-MSLN monoclonal antibody, human IgG1 (SKU#: MAB1458) with high affinity KD < 5 nM as measured by ELISA.
Molecular Mass : Calculated molecular mass (kDa): 35.9; Estimated by SDS-PAGE under reducing condition (kDa): 48-50
AA Sequence : EVEKTACPSG KKAREIDESL IFYKKWELEA CVDAALLATQ MDRVNAIPFT YEQLDVLKHK LDELYPQGYP ESVIQHLGYL FLKMSPEDIR KWNVTSLETL KALLEVNKGH EMSPQVATLI DRFVKGRGQL DKDTLDTLTA FYPGYLCSLS PEELSSVPPS SIWAVRPQDL DTCDPRQLDV LYPKARLAFQ NMNGSEYFVK IQSFLGGAPT EDLKALSQQN VSMDLATFMK LRTDAVLPLT VAEVQKLLGP HVEGLKAEER HRPVRDWILR QRQDDLDTLG LGLQGGIPNG YLVLDLSTTE NLYFQGSTGH HHHHHHH
Endotoxin : <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Purity : >90% judged by SDS-PAGE under reducing condition
Storage : The product is shipped at 4 centigrade. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below -20 centigrade for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4 centigrade for no more than 2 weeks.
Storage Buffer : Supplied at 0.5 mg/mL in sterile PBS, pH7.4 (carrier & preservative free).
Gene Name : MSLN mesothelin [ Homo sapiens (human) ]
Official Symbol : MSLN
Synonyms : MSLN; MPF; SMRP; CAK1; mesothelin; mesothelin; CAK1 antigen; megakaryocyte potentiating factor; pre-pro-megakaryocyte-potentiating factor; soluble MPF mesothelin related protein
Gene ID : 10232
mRNA Refseq : NM_001177355
Protein Refseq : NP_001170826
MIM : 601051
UniProt ID : Q13421

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