||Recombinant human XCL1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography, 12.5 kDa (114aa) (Real molecular weight on SDS-PAGE will be shift up).
||Lymphotactin, also known as XCL1, is member of gamma or C subfamily of chemokines. It is found in high levels in spleen, thymus, intestine and peripheral blood leukocytes, and at lower levels in lung, prostate gland and ovary. The expression of lymphotactin is restricted to activated T cells such as activated CD8+ T cells and other calss IMHC restricted T cells. Since lymphotactin is produced by lymphocytes and acts on lymphocytes, it is speculated that it is a messenger in T cell chemoattraction.
|Sequences of amino acids:
||MGSSHHHHHH SSGLVPRGSH MVGSEVSDKR TCVSLTTQRL PVSRIKTYTI TEGSLRAVIF ITKRGLKVCA DPQATWVRDV VRSMDRKSNT RNNMIQTKPT GTQQSTNTAV TLTG
||> 95% by SDS - PAGE
||12.5 kDa (114aa), confirmed by MALDI-TOF (molecular weight on SDS-PAGE will shift up)
||Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 30% glycerol 2 mM DTT, 0.2 M NaCl.
||0.25 mg/ml (determined by Bradford assay
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.