||Coexpression of human CDK8, amino acids M1-Y464 (as in GenBank entry NM_001260), N-terminal GST-HIS6 fusion protein with a Thrombin cleavage site and human CycC, amino acids M1-S283 (as in GenBank entry NM_005190.3), N-terminally fused to HIS6-Thrombincleavage site.
||The Cdks are serine/threonine protein kinases which are predominantly involved in the progression of the cell cycle. Subsequently, CDK-cyclin complexes (CDK7, CDK8 and CDK9) were also identified as regulators of transcription. CDK8/CycC is part of the RNA polymerase II holoenzyme complex and phosphorylates the carboxy-terminal domain (CTD) of the largest subunit of RNA polymerase II. On the other hand, CDK8/CycC phosphorylates cyclin H, a component of the CDK activating kinase (CAK), and represses activated transcription by modulation of TFIIH activity.
||Baculovirus infected Sf9 cells
||17.000 pmol/mg x min
||Theoretical MW MW GST-CDK8: 83.0 kDaTheoretical MW His-CyC: 38.0 kDa
||0.43 mg/ml (Bradford method using BSA as standard protein)
||50 mM HEPES pH 7.5, 100 mM NaCl, 5 mM DTT, 20% glycerol