Recombinant Human S100A8 & S100A9 Heterodimer Protein, His & Flag tagged S100A8 & S100A9-2217H

Recombinant Human S100A8 & S100A9 Heterodimer Protein, His & Flag tagged

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Recombinant Human S100A8 & S100A9 Heterodimer Protein, His & Flag tagged

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Cat.No. : S100A8 & S100A9-2217H
Product Overview : Human S100A8 (NP_002955.2) (Met1-Glu93), fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; Human S100A9 (NP_002956.1) (Met1-Pro114), fused with a flag tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the S100A8 & S100A9 heterodimer was purified.
Source : Baculovirus-Insect Cells
Species : Human
Tag : His & Flag
Predicted N Terminal : Met & Met
Form : Lyophilized from sterile 50 mM Tris, 500 mM NaCl, 10 % glycerol, pH 8.0. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization.
Molecular Mass : The recombinant mouse S100A8 & S100A9 heterodimer comprises 103 amino acids (S100A8) and 122 amino acids (S100A9). It has a calculated molecular mass of 12.2 kDa (S100A8) and 14.2 kDa (S100A9).
Endotoxin : < 1.0 EU per μg protein as determined by the LAL method.
Purity : > 95 % as determined by SDS-PAGE
Stability : Samples are stable for up to twelve months from date of receipt at -70ºC
Storage : Store it under sterile conditions at -20ºC to -80ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : A hardcopy of COA with reconstitution instruction is sent along with the products.
Gene Name : S100A8 S100 calcium binding protein A8 [ Homo sapiens (human) ]
Official Symbol : S100A8
Synonyms : S100A8; S100 calcium binding protein A8; MRP-8; S100 calcium-binding protein A8 (calgranulin A); calgranulin A; calgranulin-A; calprotectin L1L subunit; cystic fibrosis antigen; leukocyte L1 complex light chain; migration inhibitory factor-related protein 8; protein S100-A8; urinary stone protein band A; P8; MIF; NIF; CAGA; CFAG; CGLA; L1Ag; MRP8; CP-10; MA387; 60B8AG
Gene ID : 6279
mRNA Refseq : NM_002964
Protein Refseq : NP_002955
MIM : 123885
UniProt ID : P05109
Chromosome Location : 1q21
Function : RAGE receptor binding; Toll-like receptor 4 binding; arachidonic acid binding; calcium ion binding

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