- HIV(1)
Recombinant HIV-1 Gag p15
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| Cat.No. : | Gag p15-92H |
| Product Overview : | Recombinant HIV-1 Gag p15 was expressed in E. coli. |
| Description : | HIV-1 Gag p15 is processed by digestion of its precursor Gag p55 by HIV-1 protease. This protein is further digested into nuclocapsid protein p7 and into p6 and p1 of unknown function. This digestion is promoted by binding of HIV-1 genome RNA and the two Zn finger motifs that exist in the p7 region. The produced nucleocapsid protein p7 regulates the RNA function by directly binding to HIV-1 genome RNA. |
| Source : | E. coli |
| Species : | HIV-1 |
| Form : | 50% glycerol, 20 mM Tris-HCl (pH 7.5), 50 mM NaCl, 10 mM mercaptoethanol |
| Molecular Mass : | Its molecular size is 15 kD, same as that of p15 purified from AIDS virus particles. |
| Purity : | Over 90% by SDS-PAGE (CBB staining) |
| Applications : | 1) It can be used as a substrate for HIV-1 protease in the presence of HIV-1 genomic RNA.2) It can be used in studies of structure and function of AIDS virus as precursor of nucleocapsid p7 protein that binds to HIV-1 genome RNA.3) It can be used as p15 antigen in detection of anti-HIV-1 p15 antibody in Western blotting or ELISA.4) It can be used as a standard for the quantitative analysis of HIV-1 p15 antigen. |
| Storage : | Store at -20ºC |
| Concentration : | 0.42 mg/ml as measured by BCA method |
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