Recombinant HIV-1 Gag p15


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Recombinant HIV-1 Gag p15

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Cat.No. : Gag p15-92H
Product Overview : Recombinant HIV-1 Gag p15 was expressed in E. coli.
Description : HIV-1 Gag p15 is processed by digestion of its precursor Gag p55 by HIV-1 protease. This protein is further digested into nuclocapsid protein p7 and into p6 and p1 of unknown function. This digestion is promoted by binding of HIV-1 genome RNA and the two Zn finger motifs that exist in the p7 region. The produced nucleocapsid protein p7 regulates the RNA function by directly binding to HIV-1 genome RNA.
Source : E. coli
Species : HIV-1
Form : 50% glycerol, 20 mM Tris-HCl (pH 7.5), 50 mM NaCl, 10 mM mercaptoethanol
Molecular Mass : Its molecular size is 15 kD, same as that of p15 purified from AIDS virus particles.
Purity : Over 90% by SDS-PAGE (CBB staining)
Applications : 1) It can be used as a substrate for HIV-1 protease in the presence of HIV-1 genomic RNA.2) It can be used in studies of structure and function of AIDS virus as precursor of nucleocapsid p7 protein that binds to HIV-1 genome RNA.3) It can be used as p15 antigen in detection of anti-HIV-1 p15 antibody in Western blotting or ELISA.4) It can be used as a standard for the quantitative analysis of HIV-1 p15 antigen.
Storage : Store at -20ºC
Concentration : 0.42 mg/ml as measured by BCA method

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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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