Recombinant HIV-1 Gag p15 Gag p15-92H

Recombinant HIV-1 Gag p15

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Recombinant HIV-1 Gag p15

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Cat.No. : Gag p15-92H
Product Overview : Recombinant HIV-1 Gag p15 was expressed in E. coli.
Description : HIV-1 Gag p15 is processed by digestion of its precursor Gag p55 by HIV-1 protease. This protein is further digested into nuclocapsid protein p7 and into p6 and p1 of unknown function. This digestion is promoted by binding of HIV-1 genome RNA and the two Zn finger motifs that exist in the p7 region. The produced nucleocapsid protein p7 regulates the RNA function by directly binding to HIV-1 genome RNA.
Source : E. coli
Species : HIV-1
Form : 50% glycerol, 20 mM Tris-HCl (pH 7.5), 50 mM NaCl, 10 mM mercaptoethanol
Molecular Mass : Its molecular size is 15 kD, same as that of p15 purified from AIDS virus particles.
Purity : Over 90% by SDS-PAGE (CBB staining)
Applications : 1) It can be used as a substrate for HIV-1 protease in the presence of HIV-1 genomic RNA.2) It can be used in studies of structure and function of AIDS virus as precursor of nucleocapsid p7 protein that binds to HIV-1 genome RNA.3) It can be used as p15 antigen in detection of anti-HIV-1 p15 antibody in Western blotting or ELISA.4) It can be used as a standard for the quantitative analysis of HIV-1 p15 antigen.
Storage : Store at -20ºC
Concentration : 0.42 mg/ml as measured by BCA method

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