Recombinant Human TIGIT protein, Fc-tagged
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|Product Overview :||Recombinant Human TIGIT protein (Met22-Pro141), fused to human IgG1 Fc tag at C-terminus, was expressed in human 293 cells (HEK293).|
|Description :||T-cell immunoreceptor with Ig and ITIM domains (TIGIT) is also known as V-set and immunoglobulin domain-containing protein 9 (VSIG9), V-set and transmembrane domain-containing protein 3 (VSTM3),which belongs to single-pass type I membrane protein containing an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT is expressed at low levels on peripheral memory and regulatory CD4+ T-cells and NK cells and is up-regulated following activation of these cells (at protein level). TIGIT binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells.|
|Tag :||human IgG1 Fc|
|Form :||Lyophilized from 0.22 um filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.|
|Molecular Mass :||The protein has a calculated MW of 39.7 kDa. The protein migrates as 45-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.|
|Protein length :||120|
|Endotoxin :||Less than 1.0 EU per ug by the LAL method.|
|Purity :||>90% as determined by SDS-PAGE.|
For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 centigrade to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
|Reconstitution :||It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.|
|SDS-PAGE of TIGIT-107H :||
Human TIGIT, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
|Bioactivity-ELISA of TIGIT-107H :||
Immobilized Human CD155, Mouse IgG2a Fc Tag, low endotoxin at 5 μg/mL (100 μL/well) can bind Human TIGIT, Fc Tag with a linear range of 0.6-5 ng/mL (QC tested).
Immobilized Human TIGIT, Fc Tag at 10 μg/mL (100 μL/well) can bind Biotinylated Human Nectin-2, Fc,Avitag with a linear range of 0.313-10 μg/mL (Routinely tested).
Serial dilutions of Human TIGIT Neutralizing antibody were added into Human TIGIT, Fc Tag : Biotinylated Human CD155, Fc,Avitag binding reactions. The half maximal inhibitory concentration (IC50) is 0.55834 μg/mL (Routinely tested).
|Bioactivity-FACS of TIGIT-107H :||
FACS assay shows that recombinant Human TIGIT, Fc Tag can bind to 293T cell overexpressing human CD155. The concentration of TIGIT used is 0.1 μg/mL (Routinely tested).
|Gene Name :||TIGIT|
|Official Symbol :||TIGIT|
|Synonyms :||TIGIT; T cell immunoreceptor with Ig and ITIM domains; V set and immunoglobulin domain containing 9 , V set and transmembrane domain containing 3 , VSIG9, VSTM3; T-cell immunoreceptor with Ig and ITIM domains; DKFZp667A205; FLJ39873; V-set and transmembrane domain containing 3; V-set and immunoglobulin domain containing 9; Washington University cell adhesion molecule; V-set and transmembrane domain-containing protein 3; V-set and immunoglobulin domain-containing protein 9; VSIG9; VSTM3; WUCAM|
|Gene ID :||201633|
|mRNA Refseq :||NM_173799|
|Protein Refseq :||NP_776160|
|UniProt ID :||Q495A1|
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