Recombinant Human EGFR, His-GST tagged EGFR-31H

Recombinant Human EGFR, His-GST tagged

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Recombinant Human EGFR, His-GST tagged

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Cat.No. : EGFR-31H
Product Overview : Recombinant Human EGFR (NP_005219) cytoplasmic domain (Met 668-Ala 1210), fused with the N-terminal polyhistidine-tagged GST tag, was produced in Baculovirus-Insect cells.
Source : Baculovirus-Insect cells
Species : Human
Tag : His-GST
Predicted N Terminal : Met
Form : Supplied as sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization.
Bio-activity : The specific activity was determined to be 140 nmol/min/mg using Poly(Glu:Tyr) 4:1 as substrate (see protocol for kinase assay).
Molecular Mass : The recombinant human EGFR /GST chimera consists of 780 amino acids and has a calculated molecular mass of 89.1 kDa.
Endotoxin : <1.0 EU per μg protein as determined by the LAL method.
Purity : > 85 % as determined by SDS-PAGE
Stability : Samples are stable for up to twelve months from date of receipt at -20ºC
Storage : Store it under sterile conditions at -20ºC to -80ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping : Liquid. It is shipped out with blue ice.
Gene Name : EGFR epidermal growth factor receptor [ Homo sapiens (human) ]
Official Symbol : EGFR
Synonyms : EGFR; epidermal growth factor receptor; EC 2.7.10.1; ERBB; ERBB1; HER1; PIG61; mENA; receptor tyrosine-protein kinase erbB-1; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b) oncogene homolog); epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian); EC 2.7.10; Proto-oncogene c-ErbB-1; OTTHUMP00000159661; OTTHUMP00000159662; OTTHUMP00000159663; OTTHUMP00000209210; OTTHUMP00000209211
Gene ID : 1956
mRNA Refseq : NM_005228
Protein Refseq : NP_005219
MIM : 131550
UniProt ID : P00533
Chromosome Location : 7p12
Pathway : Adaptive Immune System; Alpha6-Beta4 Integrin Signaling Pathway; Androgen receptor signaling pathway
Function : ATP binding; MAP kinase kinase kinase activity; actin filament binding

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