||Recombinant Human UBA6 (Accession # A0AVT1) , fused with an N-terminal GST (glutathione S-transferase) tag, was produced in Spodoptera frugiperda, Sf 9 (baculovirus)-derived.
||Sf 9 insect cells
||Supplied as a solution in HEPES and DTT.
||Recombinant Human GST-Ubiquitin E1 Enzyme (UBE1L2/UBA6) is a member of the Ubiquitin-activating (E1) enzyme family that is required for the first step of the enzymatic cascade that subsequently utilizes a Ubiquitin-conjugating (E2) enzyme and a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human GST-Ubiquitin E1 Enzyme (UBE1L2/UBA6) concentration of 50-200 nM.
||Recombinant Human UBA6, GST-tagged has a calculated MW of 144 kDa.
||>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
||Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).