Active Recombinant Mouse Gzmd, His-tagged

• Cat.No.: Gzmd-170M
• Product Overview: Recombinant Mouse Gzmd (Accession # AAA37736) Ile21-Leu252, fused with a C-terminal 10-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Mouse
• Tag: His
• Predicted N Terminal: Ile21 & His25
• Form: Lyophilized from a 0.2 µm filtered solution in HEPES and NaCl.
• Bio-activity: Measured by its ability to cleave a peptide substrate, Succinyl-Phe-Leu-Phe-ThioBenzyl ester (Suc-FLF-SBzl), in the presence of 5,5"-Dithio-bis (2-nitrobenzoic acid) (DTNB). Edwards, K.M. et al. (1999) J. Biol. Chem. 274:30468.The specific activity is >50 pmol/min/µg, as measured under the described conditions.
• Molecular Mass: Recombinant Mouse Gzmd, His-tagged has a calculated MW of 27 kDa. In SDS-PAGE migrates as 42 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: Gzmd granzyme D [ Mus musculus (house mouse) ]
• Official Symbol: Gzmd
• Synonyms: Gzmd; granzyme D; CCP5; Ctla5; Ctla-5
• Gene ID: 14941
• mRNA Refseq: NM_010372
• Protein Refseq: NP_034502
• UniProt ID: P11033
• Chromosome Location: 14 C3; 14 28.19 cM