Recombinant Mouse Gzmd, His-tagged Gzmd-170M

Recombinant Mouse Gzmd, His-tagged


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Recombinant Mouse Gzmd, His-tagged

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Cat.No. : Gzmd-170M
Product Overview : Recombinant Mouse Gzmd (Accession # AAA37736) Ile21-Leu252, fused with a C-terminal 10-His tag, was produced in Mouse myeloma cell line, NS0-derived.
Source : NSO cells
Species : Mouse
Tag : His
Predicted N Terminal : Ile21 & His25
Form : Lyophilized from a 0.2 µm filtered solution in HEPES and NaCl.
Bio-activity : Measured by its ability to cleave a peptide substrate, Succinyl-Phe-Leu-Phe-ThioBenzyl ester (Suc-FLF-SBzl), in the presence of 5,5"-Dithio-bis (2-nitrobenzoic acid) (DTNB). Edwards, K.M. et al. (1999) J. Biol. Chem. 274:30468.The specific activity is >50 pmol/min/µg, as measured under the described conditions.
Molecular Mass : Recombinant Mouse Gzmd, His-tagged has a calculated MW of 27 kDa. In SDS-PAGE migrates as 42 kDa, reducing conditions.
Purity : >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage : Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Gene Name : Gzmd granzyme D [ Mus musculus (house mouse) ]
Official Symbol : Gzmd
Synonyms : Gzmd; granzyme D; CCP5; Ctla5; Ctla-5
Gene ID : 14941
mRNA Refseq : NM_010372
Protein Refseq : NP_034502
UniProt ID : P11033
Chromosome Location : 14 C3; 14 28.19 cM

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