"Gzmd" Related Products

Recombinant Mouse Gzmd, His-tagged

Cat.No.: Gzmd-170M
Product Overview: Recombinant Mouse Gzmd (Accession # AAA37736) Ile21-Leu252, fused with a C-terminal 10-His tag, was produced in Mouse myeloma cell line, NS0-derived.
Source: NSO cells
Species: Mouse
Tag: His
Predicted N Terminal: Ile21 & His25
Form: Lyophilized from a 0.2 µm filtered solution in HEPES and NaCl.
Bio-activity: Measured by its ability to cleave a peptide substrate, Succinyl-Phe-Leu-Phe-ThioBenzyl ester (Suc-FLF-SBzl), in the presence of 5,5"-Dithio-bis (2-nitrobenzoic acid) (DTNB). Edwards, K.M. et al. (1999) J. Biol. Chem. 274:30468.The specific activity is >50 pmol/min/µg, as measured under the described conditions.
Molecular Mass: Recombinant Mouse Gzmd, His-tagged has a calculated MW of 27 kDa. In SDS-PAGE migrates as 42 kDa, reducing conditions.
Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Gene Name: Gzmd granzyme D [ Mus musculus (house mouse) ]
Official Symbol: Gzmd
Synonyms: Gzmd; granzyme D; CCP5; Ctla5; Ctla-5
Gene ID: 14941
mRNA Refseq: NM_010372
Protein Refseq: NP_034502
UniProt ID: P11033
Chromosome Location: 14 C3; 14 28.19 cM

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