||Recombinant Rat Pdgfa&Pdgfb (Accession # AAB59693.1 (A chain) & Q05028.1 (B chain)) was produced in E. coli-derived.
|Predicted N Terminal:
||Ser87 (A chain) & Ser74 (B chain)
||Lyophilized from a 0.2 µm filtered solution in Acetonitrile and Ammonium Acetate.
||Measured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 10^9:749.The ED50 for this effect is typically 1 - 3 ng/mL.
||Recombinant Rat Pdgfa&Pdgfb has a calculated MW of 24.8 kDa.
||>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
||Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).