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Recombinant Human SFRP1, His-tagged

Cat.No.: SFRP1-221H
Product Overview: Recombinant Human SFRP1 (Accession # AAB70793) Ser32-Lys314 , fused with a C-terminal 6-His tag, was produced in Mouse myeloma cell line, NS0-derived.
Source: NSO cells
Species: Human
Tag: His
Predicted N Terminal: Ser32 & Phe39 & Asp42
Form: Lyophilized from a 0.2 µm filtered solution in NaH2PO4 and NaCl with BSA as a carrier protein.
Bio-activity: Measured by its ability to inhibit proliferation of HeLa human cervical epithelial carcinoma cells. Ko, L. et al. (2002) Exp. Cell Res. 280:280.The ED50 for this effect is typically 2.5 - 10 µg/mL.
Molecular Mass: Recombinant Human SFRP1, His-tagged has a calculated MW of 33 kDa. In SDS-PAGE migrates as 35-40 kDa, reducing conditions.
Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Reconstitution: Reconstitute at 200 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Gene Name: SFRP1 secreted frizzled-related protein 1 [ Homo sapiens ]
Official Symbol: SFRP1
Synonyms: SFRP1; secreted frizzled-related protein 1; FRP; FRP 1; SARP2; SARP-2; sFRP-1; secreted apoptosis-related protein 2; FRP1; FrzA; FRP-1;
Gene ID: 6422
mRNA Refseq: NM_003012
Protein Refseq: NP_003003
MIM: 604156
UniProt ID: Q8N474
Chromosome Location: 8p11.21

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