Active Recombinant Human SFRP1

• Cat.No.: SFRP1-222H
• Product Overview: Recombinant Human SFRP1 (Accession # AAB70793) Ser32-Lys314 was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Human
• Predicted N Terminal: Ser32, Phe39 (minor) & Asp42 (minor)
• Form: Lyophilized from a 0.2 µm filtered solution in Phosphate and NaCl.
• Bio-activity: Measured by its ability to inhibit proliferation of HeLa human cervical epithelial carcinoma cells. Ko, L. et al. (2002) Exp. Cell Res. 280:280.The ED50 for this effect is typically 0.15-0.75 µg/mL.
• Molecular Mass: Recombinant Human SFRP1 has a calculated MW of 32.6 kDa. In SDS-PAGE migrates as 33-40 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: SFRP1 secreted frizzled-related protein 1 [ Homo sapiens ]
• Official Symbol: SFRP1
• Synonyms: SFRP1; secreted frizzled-related protein 1; FRP; FRP 1; SARP2; SARP-2; sFRP-1; secreted apoptosis-related protein 2; FRP1; FrzA; FRP-1
• Gene ID: 6422
• mRNA Refseq: NM_003012
• Protein Refseq: NP_003003
• MIM: 604156
• UniProt ID: Q8N474
• Chromosome Location: 8p11.21