Recombinant Mouse Sfrp2, His-tagged

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Recombinant Mouse Sfrp2, His-tagged

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Cat.No. : Sfrp2-223M
Product Overview : Recombinant Mouse Sfrp2 (Accession # AAB70795) Leu25-Cys295, fused with a C-terminal 6-His tag, was produced in Mouse myeloma cell line, NS0-derived.
Source : NSO cells
Species : Mouse
Tag : His
Predicted N Terminal : Leu25
Form : Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
Bio-activity : Measured by its ability to compete with Frizzled-1 for binding to biotinylated Wnt-3a.The IC50 value is 0.7-3.5 nM, under conditions in which Recombinant Mouse (rm) Frizzled-1 Fc Chimera is present at 2.1 nM, and biotinylated rmWnt-3a concentration is 2.7 nM.
Molecular Mass : Recombinant Mouse Sfrp2, His-tagged has a calculated MW of 31.6 kDa. In SDS-PAGE migrates as 35-40 kDa, reducing conditions.
Purity : >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage : Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Reconstitution : Reconstitute at 100 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Gene Name : Sfrp2 secreted frizzled-related protein 2 [ Mus musculus ]
Official Symbol : Sfrp2
Synonyms : SFRP2; secreted frizzled-related protein 2; SARP-1; sFRP-2; stromal cell derived factor 5; secreted apoptosis-related protein 1; secreted frizzled-related sequence protein 2; secreted frizzled-related sequence protein 5; Sdf5; AI851596;
Gene ID : 20319
mRNA Refseq : NM_009144
Protein Refseq : NP_033170
MIM :
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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