Active Recombinant Mouse Smoc1, His-tagged

• Cat.No.: Smoc1-272M
• Product Overview: Recombinant Mouse Smoc1 (Accession # AAD41590) His26-Val452, fused with a C-terminal 6-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Mouse
• Tag: His
• Predicted N Terminal: His26 & Ile35
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of A431 human epithelial carcinoma cells.When 5 x 10^4 cells/well are added to rmSMOC-1 coated plates, cell adhesion is enhanced in a dose dependent manner after 75 minutes at 37 °C. The ED50 for this effect is typically 1 - 4 µg/mL.
• Molecular Mass: Recombinant Mouse Smoc1, His-tagged has a calculated MW of 47.9 kDa. In SDS-PAGE migrates as 60-65 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: Smoc1 SPARC related modular calcium binding 1 [ Mus musculus ]
• Official Symbol: Smoc1
• Synonyms: SMOC1; SPARC related modular calcium binding 1; SPARC-related modular calcium-binding protein 1; SMOC-1; SPARC-related protein; SPARC-related gene protein; secreted modular calcium binding protein 1; secreted modular calcium-binding protein 1; SRG; AI848508; 2600002F22Rik
• Gene ID: 64075
• mRNA Refseq: NM_001146217
• Protein Refseq: NP_001139689