Recombinant Mouse Smoc1, His-tagged Smoc1-272M

Recombinant Mouse Smoc1, His-tagged


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Recombinant Mouse Smoc1, His-tagged

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Cat.No. : Smoc1-272M
Product Overview : Recombinant Mouse Smoc1 (Accession # AAD41590) His26-Val452, fused with a C-terminal 6-His tag, was produced in Mouse myeloma cell line, NS0-derived.
Source : NSO cells
Species : Mouse
Tag : His
Predicted N Terminal : His26 & Ile35
Form : Lyophilized from a 0.2 µm filtered solution in PBS.
Bio-activity : Measured by the ability of the immobilized protein to support the adhesion of A431 human epithelial carcinoma cells.When 5 x 10^4 cells/well are added to rmSMOC-1 coated plates, cell adhesion is enhanced in a dose dependent manner after 75 minutes at 37 °C. The ED50 for this effect is typically 1 - 4 µg/mL.
Molecular Mass : Recombinant Mouse Smoc1, His-tagged has a calculated MW of 47.9 kDa. In SDS-PAGE migrates as 60-65 kDa, reducing conditions.
Purity : >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage : Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Gene Name : Smoc1 SPARC related modular calcium binding 1 [ Mus musculus ]
Official Symbol : Smoc1
Synonyms : SMOC1; SPARC related modular calcium binding 1; SPARC-related modular calcium-binding protein 1; SMOC-1; SPARC-related protein; SPARC-related gene protein; secreted modular calcium binding protein 1; secreted modular calcium-binding protein 1; SRG; AI848508; 2600002F22Rik;
Gene ID : 64075
mRNA Refseq : NM_001146217
Protein Refseq : NP_001139689

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