Recombinant Human ASAH2, His-tagged ASAH2-284H

Recombinant Human ASAH2, His-tagged


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Recombinant Human ASAH2, His-tagged

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Cat.No. : ASAH2-284H
Product Overview : Recombinant Human ASAH2 (Accession # AAF86240) Thr15-Ile761, fused with an N-terminal 6-His tag, was produced in Chinese Hamster Ovary cell line, CHO-derived.
Source : CHO cells
Species : Human
Tag : His
Predicted N Terminal : His
Form : Supplied as a 0.2 µm filtered solution in MES, NaCl and Sodium Cholate.
Bio-activity : Measured by its ability to hydrolyze the substrate C12:0 ceramide into sphingosine and dodecanoic acid. The specific activity is >5,000 pmol/min/µg, as measured under the described conditions.
Molecular Mass : Recombinant Human ASAH2, His-tagged has a calculated MW of 83 kDa. In SDS-PAGE migrates as 132 kDa, reducing conditions.
Purity : >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage : Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Gene Name : ASAH2 N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2 [ Homo sapiens ]
Official Symbol : ASAH2
Synonyms : ASAH2; N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2; neutral ceramidase; mitochondrial ceramidase; non-lysosomal ceramidase; acylsphingosine deacylase 2; neutral/alkaline ceramidase; N-acylsphingosine amidohydrolase 2; HNAC1; BCDase; LCDase; NCDase; N-CDase;
Gene ID : 56624
mRNA Refseq : NM_001143974
Protein Refseq : NP_001137446
MIM : 611202
UniProt ID : Q9NR71
Chromosome Location : 10q11.23

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