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Recombinant Human ASAH2, His-tagged

Cat.No.: ASAH2-284H
Product Overview: Recombinant Human ASAH2 (Accession # AAF86240) Thr15-Ile761, fused with an N-terminal 6-His tag, was produced in Chinese Hamster Ovary cell line, CHO-derived.
Source: CHO cells
Species: Human
Tag: His
Predicted N Terminal: His
Form: Supplied as a 0.2 µm filtered solution in MES, NaCl and Sodium Cholate.
Bio-activity: Measured by its ability to hydrolyze the substrate C12:0 ceramide into sphingosine and dodecanoic acid. The specific activity is >5,000 pmol/min/µg, as measured under the described conditions.
Molecular Mass: Recombinant Human ASAH2, His-tagged has a calculated MW of 83 kDa. In SDS-PAGE migrates as 132 kDa, reducing conditions.
Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Gene Name: ASAH2 N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2 [ Homo sapiens ]
Official Symbol: ASAH2
Synonyms: ASAH2; N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2; neutral ceramidase; mitochondrial ceramidase; non-lysosomal ceramidase; acylsphingosine deacylase 2; neutral/alkaline ceramidase; N-acylsphingosine amidohydrolase 2; HNAC1; BCDase; LCDase; NCDase; N-CDase;
Gene ID: 56624
mRNA Refseq: NM_001143974
Protein Refseq: NP_001137446
MIM: 611202
UniProt ID: Q9NR71
Chromosome Location: 10q11.23

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