Recombinant Human POMGNT1, His-tagged POMGNT1-289H

Recombinant Human POMGNT1, His-tagged

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Recombinant Human POMGNT1, His-tagged

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Cat.No. : POMGNT1-289H
Product Overview : Recombinant Human POMGNT1 (Accession # AAH01471) Ser66-Thr660, fused with an N-terminal human CD33 signal sequence and a C-terminal 6-His tag, was produced in Spodoptera frugiperda, Sf 21 (baculovirus)-derived.
Source : Baculovirus Sf 21 Insect cells
Species : Human
Tag : His
Predicted N Terminal : Ser66
Form : Supplied as a 0.2 µm filtered solution in Tris, NaCl and Glycerol.
Bio-activity : Measured by its ability to transfer N-Acetyl-D-glucosamine from UDP-GlcNAc to Methyl-alpha -D-mannopyronoside.The specific activity is >500 pmol/min/µg, as measured under the described conditions.
Molecular Mass : Recombinant Human POMGNT1, His-tagged has a calculated MW of 68 kDa. In SDS-PAGE migrates as 65-80 kDa, reducing conditions.
Purity : >90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 µg per lane.
Storage : Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Gene Name : POMGNT1 protein O-linked mannose beta1,2-N-acetylglucosaminyltransferase [ Homo sapiens ]
Official Symbol : POMGNT1
Synonyms : POMGNT1; protein O-linked mannose beta1,2-N-acetylglucosaminyltransferase; protein O-linked-mannose beta-1,2-N-acetylglucosaminyltransferase 1; FLJ20277; MGAT1.2; protein O mannose beta 1; 2 N acetylglucosaminyltransferase; UDP-GlcNAc:alpha-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I.2; MEB; GNTI.2; MDDGA3; MDDGB3; MDDGC3; GnT I.2; gnT-I.2; DKFZp761B182;
Gene ID : 55624
mRNA Refseq : NM_001243766
Protein Refseq : NP_001230695
MIM : 606822
UniProt ID : Q8WZA1
Chromosome Location : 1p34.1

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