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Recombinant Human MSLN, His-tagged

Cat.No.: MSLN-301H
Product Overview: Recombinant Human MSLN (Accession # AAH09272) Glu296-Gly580, fused with a C-terminal 6-His tag, was produced in Mouse myeloma cell line, NS0-derived.
Source: NSO cells
Species: Human
Tag: His
Predicted N Terminal: Glu296
Form: Lyophilized from a 0.2 µm filtered solution in PBS with Trehalose.
Bio-activity: Measured by its binding ability in a functional ELISA.When Recombinant Human CA125/MUC16 is coated at 2 µg/mL (100 µL/well), the concentration of Recombinant Human Mesothelin that produces 50% of the optimal binding response is found to be approximately 0.5 - 2.5 µg/mL.
Molecular Mass: Recombinant Human MSLN, His-tagged has a calculated MW of 33 kDa. In SDS-PAGE migrates as 40-45 kDa, reducing conditions.
Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Gene Name: MSLN mesothelin [ Homo sapiens ]
Official Symbol: MSLN
Synonyms: MSLN; mesothelin; CAK1; MPF; CAK1 antigen; megakaryocyte potentiating factor; soluble MPF mesothelin related protein; pre-pro-megakaryocyte-potentiating factor; SMRP;
Gene ID: 10232
mRNA Refseq: NM_001177355
Protein Refseq: NP_001170826
MIM: 601051
UniProt ID: Q13421
Chromosome Location: 16p13.3

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