Active Recombinant Mouse Wnt5b

• Cat.No.: Wnt5b-303M
• Product Overview: Recombinant Mouse Wnt5b (Accession # AAH10775) Gln31-Lys372 was produced in Chinese Hamster Ovary cell line, CHO-derived.

Specification

• Source: CHO cells
• Species: Mouse
• Predicted N Terminal: No results obtained: Gln31 predicted
• Form: Lyophilized from a 0.2 µm filtered solution in PBS, EDTA and CHAPS with BSA as a carrier protein.
• Bio-activity: Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3-E1 mouse preosteoblast cells.The ED50 for this effect is typically 0.1-0.5 µg/mL, in the presence of 5 ng/mL of Recombinant Mouse Wnt-3a. Optimal dilutions should be determined by each laboratory for each application.
• Molecular Mass: Recombinant Mouse Wnt5b has a calculated MW of 38.6 kDa. In SDS-PAGE migrates as 45 kDa, reducing conditions.
• Purity: >75%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 100 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: Wnt5b wingless-related MMTV integration site 5B [ Mus musculus ]
• Official Symbol: Wnt5b
• Synonyms: WNT5B; wingless-related MMTV integration site 5B; protein Wnt-5b; Wnt-5b; AW545702
• Gene ID: 22419
• mRNA Refseq: NM_009525
• Protein Refseq: NP_033551