Active Recombinant Mouse Smoc2, His-tagged

• Cat.No.: Smoc2-311M
• Product Overview: Recombinant Mouse Smoc2 (Accession # AAH19527) Leu30-Gly447, fused with a C-terminal 6-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Mouse
• Tag: His
• Predicted N Terminal: Leu30
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of A431 human epithelial carcinoma cells.When 5 x 10^4 cells/well are added to rmSMOC-2 coated plates, cell adhesion is enhanced in a dose dependent manner after 75 minutes at 37 °C. The ED50 for this effect is typically 0.2 - 0.8 µg/mL.
• Molecular Mass: Recombinant Mouse Smoc2, His-tagged has a calculated MW of 47.6 kDa. In SDS-PAGE migrates as 58-61 kDa, reducing conditions.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: Smoc2 SPARC related modular calcium binding 2 [ Mus musculus ]
• Official Symbol: Smoc2
• Synonyms: SMOC2; SPARC related modular calcium binding 2; SPARC-related modular calcium-binding protein 2; SMOC-2; secreted modular calcium-binding protein 2; Smoc2l; 1700056C05Rik; 5430426J21Rik
• Gene ID: 64074
• mRNA Refseq: NM_022315
• Protein Refseq: NP_071710