Active Recombinant Human PADI4, His-tagged

• Cat.No.: PADI4-317H
• Product Overview: Recombinant Human PADI4 (Accession # AAH25718) Ala2-Pro663, fused with an N-terminal 11 kDa fusion protein, a thrombin cleavage linker and a C-terminal 6-His tag, was produced in E. coli-derived.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Predicted N Terminal: Inconclusive result, Met predicted. Protein identity confirmed by MS analysis of tryptic fragments
• Form: Supplied as a 0.2 µm filtered solution in Sodium Acetate, NaCl, DTT, EDTA and Glycerol.
• Bio-activity: Measured by its ability to catalyze the deimination of benzoyl-arginine ethyl ester.The specific activity is >1000 pmol/min/µg, as measured under the described conditions.
• Molecular Mass: Recombinant Human PADI4, His-tagged has a calculated MW of 87 kDa. In SDS-PAGE migrates as 75-85 kDa, reducing conditions.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 µg per lane.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: PADI4 peptidyl arginine deiminase, type IV [ Homo sapiens ]
• Official Symbol: PADI4
• Synonyms: PADI4; peptidyl arginine deiminase, type IV; PADI5, peptidyl arginine deiminase, type V; protein-arginine deiminase type-4; PAD; PDI4; PDI5; HL-60 PAD; PADI-H protein; protein-arginine deiminase type IV; peptidyl arginine deiminase, type V; PAD4; PADI5
• Gene ID: 23569
• mRNA Refseq: NM_012387
• Protein Refseq: NP_036519
• MIM: 605347
• UniProt ID: Q9UM07
• Chromosome Location: 1p36.13