Recombinant Mouse Col13a1, His-tagged Col13a1-325M

Recombinant Mouse Col13a1, His-tagged


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Recombinant Mouse Col13a1, His-tagged

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Cat.No. : Col13a1-325M
Product Overview : Recombinant Mouse Col13a1 (Accession # AAH34164) Glu107-Gln565, fused with an N-terminal 6-His tag, was produced in Mouse myeloma cell line, NS0-derived.
Source : NSO cells
Species : Mouse
Tag : His
Predicted N Terminal : His
Form : Lyophilized from a 0.2 µm filtered solution in PBS.
Bio-activity : Measured by the ability of the immobilized protein to support the adhesion of A431 human epithelial carcinoma cells.When 1 x 10^5 cells per well are added to rmCOL-13A1 coated plate (2 µg/mL, 100 µL/well), approximately 45% - 75% will adhere after 1 hour at 37 °C. Optimal dilutions should be determined by each laboratory for each application.
Molecular Mass : Recombinant Mouse Col13a1, His-tagged has a calculated MW of 46.0 kDa. In SDS-PAGE migrates as 50-60 kDa, reducing conditions.
Purity : >80%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage : Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Gene Name : Col13a1 collagen, type XIII, alpha 1 [ Mus musculus ]
Official Symbol : Col13a1
Synonyms : COL13A1; collagen, type XIII, alpha 1; collagen alpha-1(XIII) chain; type XIII collagen; procollagen, type XIII, alpha 1;
Gene ID : 12817
mRNA Refseq : NM_007731
Protein Refseq : NP_031757

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