Active Recombinant Human HDAC8, Met&His-tagged

• Cat.No.: HDAC8-335H
• Product Overview: Recombinant Human HDAC8 (Accession # AAH50433) Met1-Val377, fused with an N-terminal Met and 7-His tag, was produced in Spodoptera frugiperda, Sf 21 (baculovirus)-derived.

Specification

• Species: Human
• Source: Sf21 Cells
• Tag: His
• Protein Length: 1-377 a.a.
• Predicted N Terminal: No results obtained: Met predicted
• Form: Supplied as a 0.2 µm filtered solution in Tris, NaCl and Glycerol.
• Bio-activity: Measured by its ability to remove the acetyl group from a fluorogenic peptide substrate Ac-RGK(Ac)-AMC in a coupled assay.The specific activity is >1.5 pmol/min/µg, as measured under the described conditions.
• Molecular Mass: Recombinant Human HDAC8, Met&His-tagged has a calculated MW of 43 kDa. In SDS-PAGE migrates as 48 kDa, reducing conditions.
• Purity: >85%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Publications:
  Circumvention of Gefitinib Resistance by Repurposing Flunarizine via Histone Deacetylase Inhibition (2023)

Gene Information

• Gene Name: HDAC8 histone deacetylase 8 [ Homo sapiens ]
• Official Symbol: HDAC8
• Synonyms: HDAC8; histone deacetylase 8; HDACL1, histone deacetylase like 1; RPD3; histone deacetylase-like 1; HD8; CDA07; HDACL1
• Gene ID: 55869
• mRNA Refseq: NM_001166418
• Protein Refseq: NP_001159890
• MIM: 300269
• UniProt ID: Q9BY41
• Chromosome Location: Xq13