Active Recombinant Human RGMB protein, His-tagged

• Cat.No.: RGMB-345H
• Product Overview: Recombinant Human RGMB(Gly87-Asp209 (N-terminus chain) & Pro210-Ser452 (C-terminus chain)) fused with His tag at C-terminal was expressed in NS0.

Specification

• Source: Mammalian cells
• Species: Human
• Tag: His
• Predicted N Terminal: Gly87 & Pro210
• Form: Lyophilized from a 0.2 μm filtered solution in PBS.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immoblized rhBMP-4 at 1 µg/mL (100 µL/well) can bind rhRGM-B with a linear range of 10-500 ng/mL.
• Molecular Mass: Predicted Molecular Mass: 13.4 (N-terminus chain), 27.7 (C-terminus chain) kDa;SDS-PAGE: 20 kDa and 35 kDa, reducing conditions.
• Protein length: Gly87-Asp209 (N-terminus chain) & Pro210-Ser452 (C-terminus chain)
• Endotoxin: <0.01 EU per 1 μg of the protein by the LAL method.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Storage: Avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 centigrade as supplied.1 month, 2 to 8 centigrade under sterile conditions after reconstitution.3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 100 μg/mL in sterile PBS.

Gene Information

• Gene Name: RGMB RGM domain family, member B [ Homo sapiens ]
• Official Symbol: RGMB
• Synonyms: RGMB; RGM domain family, member B; RGM domain family member B; FLJ90406; repulsive guidance molecule B; DRG11-responsive axonal guidance and outgrowth of neurite; DRAGON; MGC86970; DKFZp434P228
• Gene ID: 285704
• mRNA Refseq: NM_001012761
• Protein Refseq: NP_001012779
• MIM: 612687
• UniProt ID: Q6NW40
• Chromosome Location: 5q21.1
• Pathway: Axon guidance, organism-specific biosystem; BMP receptor signaling, organism-specific biosystem; Developmental Biology, organism-specific biosystem; Netrin-1 signaling, organism-specific biosystem
• Function: identical protein binding