Recombinant Human FGF8, Met-tagged FGF8-348H

Recombinant Human FGF8, Met-tagged

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Recombinant Human FGF8, Met-tagged

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Cat.No. : FGF8-348H
Product Overview : Recombinant Human FGF8 (Accession # AAH69106) Gln23-Arg244, fused with an N-terminal Met, was produced in E. coli-derived.
Source : E. coli
Species : Human
Predicted N Terminal : Met
Form : Lyophilized from a 0.2 µm filtered solution in MOPS, Na2SO4, EDTA and DTT with BSA as a carrier protein.
Bio-activity : Measured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cells. Rizzino, A. et al. (1988) Cancer Res. 48:4266; Thomas, K. et al. (1987) Methods Enzymol. 147:120.The ED50 for this effect is typically 0.04 - 0.2 µg/mL in the presence of 10 µg/mL of heparin.
Molecular Mass : Recombinant Human FGF8, Met-tagged has a calculated MW of 25.5 kDa. In SDS-PAGE migrates as 29 kDa, reducing conditions.
Purity : >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage : Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Reconstitution : Reconstitute at 100 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Gene Name : FGF8 fibroblast growth factor 8 (androgen-induced) [ Homo sapiens ]
Official Symbol : FGF8
Synonyms : FGF8; fibroblast growth factor 8 (androgen-induced); fibroblast growth factor 8; AIGF; androgen-induced growth factor; heparin-binding growth factor 8; KAL6; FGF-8; HBGF-8; MGC149376;
Gene ID : 2253
mRNA Refseq : NM_001206389
Protein Refseq : NP_001193318
MIM : 600483
UniProt ID : P55075
Chromosome Location : 10q25-q26

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