Recombinant Mouse Mepe, His-tagged Mepe-374M

Recombinant Mouse Mepe, His-tagged


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Recombinant Mouse Mepe, His-tagged

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Cat.No. : Mepe-374M
Product Overview : Recombinant Mouse Mepe (Accession # AAK70342) Met1-Asp433, fused with a C-terminal 10-His tag, was produced in Mouse myeloma cell line, NS0-derived.
Source : NSO cells
Species : Mouse
Tag : His
Predicted N Terminal : Ser147
Form : Lyophilized from a 0.2 µm filtered solution in PBS.
Bio-activity : Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells.The ED50 for this effect is typically 0.1 - 0.4 µg/mL. Optimal dilutions should be determined by each laboratory for each application.
Molecular Mass : Recombinant Mouse Mepe, His-tagged has a calculated MW of 31.6 kDa. In SDS-PAGE migrates as 40-55 kDa, reducing conditions.
Purity : >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage : Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Gene Name : Mepe matrix extracellular phosphoglycoprotein with ASARM motif (bone) [ Mus musculus ]
Official Symbol : Mepe
Synonyms : MEPE; matrix extracellular phosphoglycoprotein with ASARM motif (bone); matrix extracellular phosphoglycoprotein with ASARM motif; osteoregulin; Of45;
Gene ID : 94111
mRNA Refseq : NM_053172
Protein Refseq : NP_444402

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