Active Recombinant Mouse Mepe, His-tagged

• Cat.No.: Mepe-374M
• Product Overview: Recombinant Mouse Mepe (Accession # AAK70342) Met1-Asp433, fused with a C-terminal 10-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Mouse
• Tag: His
• Predicted N Terminal: Ser147
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells.The ED50 for this effect is typically 0.1 - 0.4 µg/mL. Optimal dilutions should be determined by each laboratory for each application.
• Molecular Mass: Recombinant Mouse Mepe, His-tagged has a calculated MW of 31.6 kDa. In SDS-PAGE migrates as 40-55 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: Mepe matrix extracellular phosphoglycoprotein with ASARM motif (bone) [ Mus musculus ]
• Official Symbol: Mepe
• Synonyms: MEPE; matrix extracellular phosphoglycoprotein with ASARM motif (bone); matrix extracellular phosphoglycoprotein with ASARM motif; osteoregulin; Of45
• Gene ID: 94111
• mRNA Refseq: NM_053172
• Protein Refseq: NP_444402