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Recombinant Human CDSN, His-tagged

Cat.No.: CDSN-407H
Product Overview: Recombinant Human CDSN (Accession # AAN70994) Lys33-Pro528, fused with a C-terminal 6-His tag (Asp526Asn), was produced in Mouse myeloma cell line, NS0-derived.
Source: NSO cells
Species: Human
Tag: His
Predicted N Terminal: Lys33
Form: Lyophilized from a 0.2 µm filtered solution in PBS.
Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of BUD-8 human fibroblast cells.When 3 x 10^4 cells/well are added to rhCDSN coated plates (10 µg/mL, 100 µL/well), approximately 50 - 80% will adhere after 1 hour at 37 °C. Optimal dilutions should be determined by each laboratory for each application.
Molecular Mass: Recombinant Human CDSN, His-tagged has a calculated MW of 49.1 kDa. In SDS-PAGE migrates as 60 kDa, reducing conditions.
Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Gene Name: CDSN corneodesmosin [ Homo sapiens ]
Official Symbol: CDSN
Synonyms: CDSN; corneodesmosin; D6S586E; differentiated keratinocyte S protein; S; PSS; HTSS; HTSS1;
Gene ID: 1041
mRNA Refseq: NM_001264
Protein Refseq: NP_001255
MIM: 602593
UniProt ID: Q15517
Chromosome Location: 6p21.3

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