Active Recombinant Mouse Vtcn1, Fc Chimera

• Cat.No.: Vtcn1-410M
• Product Overview: Recombinant Mouse Vtcn1 (Accession # AAP37284) was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Mouse
• Tag: Fc Chimera
• Predicted N Terminal: Phe29
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated human T cells.Human T lymphocytes cultured for 72 hours with PHA were incubated for an additional 3 days in 96 well plates coated with 500 ng/mL anti-CD3 and Recombinant Mouse B7-H4 Fc Chimera. The ED50 for this effect is typically 0.5 - 3 µg/mL.Optimal dilutions should be determined by each laboratory for each application.
• Molecular Mass: Recombinant Mouse Vtcn1, Fc Chimera has a calculated MW of 52.4 kDa (monomer). In SDS-PAGE migrates as 75-85 kDa, reducing conditions.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: Vtcn1 V-set domain containing T cell activation inhibitor 1 [ Mus musculus ]
• Official Symbol: Vtcn1
• Synonyms: VTCN1; V-set domain containing T cell activation inhibitor 1; V-set domain containing T-cell activation inhibitor 1; t cell costimulatory molecule B7x; immune costimulatory protein B7-H4; B7x; B7h4; B7s1; BC032925; MGC41287
• Gene ID: 242122
• mRNA Refseq: NM_178594
• Protein Refseq: NP_848709