Active Recombinant Human LY96 protein, His-tagged

• Cat.No.: LY96-445H
• Product Overview: Recombinant Human LY96(Glu17 - Asn160) fused with His tag at C-terminal was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Predicted N Terminal: Met
• Form: Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
• Bio-activity: Measured by its binding ability in a functional ELISA. When recombinant human TLR-4 is Immobilized at 2 µg/mL (100 µL/well), the concentration of rhMD-2 that produces 50% optimal binding response is found to be approximately 0.03-0.15 µg/mL.
• Molecular Mass: Predicted Molecular Mass: 19.2 (monomer) kDa
• Protein length: Glu17 - Asn160
• Endotoxin: <0.10 EU per 1 μg of the protein by the LAL method.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 centigrade as supplied.1 month, 2 to 8 centigrade under sterile conditions after reconstitution.3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Gene Information

• Gene Name: LY96 lymphocyte antigen 96 [ Homo sapiens ]
• Official Symbol: LY96
• Synonyms: LY96; lymphocyte antigen 96; MD 2; protein MD-2; myeloid differentiation protein-2; MD2; MD-2; ly-96; ESOP-1
• Gene ID: 23643
• mRNA Refseq: NM_001195797
• Protein Refseq: NP_001182726
• MIM: 605243
• UniProt ID: Q9Y6Y9
• Chromosome Location: 8q13.3
• Pathway: Activated TLR4 signalling, organism-specific biosystem; Activation of IRF3/IRF7 mediated by TBK1/IKK epsilon, organism-specific biosystem; IKK complex recruitment mediated by RIP1, organism-specific biosystem; IRAK2 mediated activation of TAK1 complex, organism-specific biosystem; Immune System, organism-specific biosystem; Innate Immune System, organism-specific biosystem; MyD88 cascade initiated on plasma membrane, organism-specific biosystem
• Function: coreceptor activity; lipopolysaccharide receptor activity; protein binding