||Recombinant Human SUMO1 (Accession # NM_003352) , fused with an N-terminal HA (YPYDVPDYA) tag and a C-terminal Vinyl Sulfone, was produced in E. coli-derived.
||Supplied as a solution in HEPES, NaCl, Glycerol.
||Add Recombinant Human HA-SUMO1 Vinyl Sulfone to in vitro assays to inhibit SUMO-specific isopeptidases (SENPs). The HA-tag allows for detection and purification of SUMO-specific isopeptidases (SENPs) activity. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human HA-SUMO1 Vinyl Sulfone concentration of 1-5 µM.
||Recombinant Human SUMO1, HA (YPYDVPDYA)-tagged has a calculated MW of 12 kDa.
||>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
||Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).