||Recombinant Human MAP3K8 (Accession # NM_005204) amino acids 30 - 397, fused with an N-terminal GST tag, was produced in baculovirus in Sf 9 insect cells.
||Sf 9 insect cells
||Supplied in 50 mM Tris-HCl (pH 7.5), 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, and 25% glycerol.
||The specific activity of COT was determined to be 1150 nmol/min/mg using MEK1 and ERK1 substrates and a myelin basic protein (MBP) substrate
||In SDS-PAGE migrates as 70 kDa.
||The purity was determined to be >90% by densitometry
||Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).