Recombinant Human ITK protein(Arg352-Leu620), GST-tagged
Cat.No. : | ITK-576H |
Product Overview : | Recombinant Human ITK (NP_005537.3) (Arg352-Leu620) was expressed in Insect Cells with a GST tag at the N-terminus. |
- Specification
- Gene Information
- Related Products
Source : | Insect Cells |
Species : | Human |
Tag : | N-GST |
Protein length : | Arg352-Leu620 |
Form : | Lyophilized from sterile 50 mM Tris, 150 mM NaCl, pH 7.5, 25 % glycerol, 0.5 mM PMSF, 0.5 mM TCEP, 0.1 mM EDTA, 0.5 mM GSH. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
Molecular Mass : | The recombinant human ITK consists of 501 amino acids and predicts a molecular mass of 57.7 kDa. |
Endotoxin : | < 1.0 EU per μg protein as determined by the LAL method. |
Purity : | > 75 % as determined by SDS-PAGE. |
Storage : | Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
Gene Name : | ITK IL2-inducible T-cell kinase [ Homo sapiens ] |
Official Symbol : | ITK |
Synonyms : | ITK; IL2-inducible T-cell kinase; tyrosine-protein kinase ITK/TSK; EMT; LYK; PSCTK2; kinase EMT; T-cell-specific kinase; tyrosine-protein kinase LYK; IL-2-inducible T cell kinase; IL-2-inducible T-cell kinase; homolog of mouse T-cell itk/tsk; interleukin-2-inducible T cell kinase; interleukin-2-inducible T-cell kinase; MGC126257; MGC126258; |
Gene ID : | 3702 |
mRNA Refseq : | NM_005546 |
Protein Refseq : | NP_005537 |
MIM : | 186973 |
UniProt ID : | Q08881 |
Products Types
◆ Recombinant Protein | ||
ITK-1311H | Recombinant Human ITK Protein (M1-L620), Tag Free | +Inquiry |
ITK-2640H | Recombinant Human ITK Protein, MYC/DDK-tagged | +Inquiry |
ITK-4961H | Active Recombinant Human ITK Protein | +Inquiry |
ITK-4960H | Recombinant Human ITK Protein, GST-tagged | +Inquiry |
Itk-3615M | Recombinant Mouse Itk Protein, Myc/DDK-tagged | +Inquiry |
◆ Lysates | ||
ITK-418MCL | Recombinant Mouse ITK cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (5)
Ask a questionITK's involvement in Th2 cell differentiation links it to allergic diseases. Dysregulation of ITK is implicated in autoimmune diseases, influencing aberrant T-cell responses and immune system dysfunction.
Deficiencies in ITK function are associated with immunodeficiency disorders. Genetic variations in ITK may compromise its kinase activity, leading to impaired T-cell signaling and immune responses.
ITK is a tyrosine kinase involved in T-cell signaling, specifically in the activation of T-cell receptors. It plays a critical role in the regulation of immune responses and T-cell-mediated immunity.
ITK is activated by phosphorylation and participates in downstream signaling pathways, including the PLCγ1 pathway, leading to the mobilization of calcium ions and activation of NFAT transcription factors.
ITK contributes to immune synapse formation by mediating cytoskeletal changes and promoting T-cell receptor clustering. This influences T-cell activation and the subsequent effector functions.
Customer Reviews (3)
Write a reviewResearch-grade excellence. This product has simplified our experiments, and the quality is unmatched.
The shipping speed surprised me! Got what I needed for our experiments right on schedule.
Customer service that goes above and beyond. They truly understand our lab's needs
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