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Recombinant Human ITK protein(Arg352-Leu620), GST-tagged

Cat.No. : ITK-576H
Product Overview : Recombinant Human ITK (NP_005537.3) (Arg352-Leu620) was expressed in Insect Cells with a GST tag at the N-terminus.
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  • Gene Information
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Source : Insect Cells
Species : Human
Tag : N-GST
Protein length : Arg352-Leu620
Form : Lyophilized from sterile 50 mM Tris, 150 mM NaCl, pH 7.5, 25 % glycerol, 0.5 mM PMSF, 0.5 mM TCEP, 0.1 mM EDTA, 0.5 mM GSH. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Molecular Mass : The recombinant human ITK consists of 501 amino acids and predicts a molecular mass of 57.7 kDa.
Endotoxin : < 1.0 EU per μg protein as determined by the LAL method.
Purity : > 75 % as determined by SDS-PAGE.
Storage : Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
Gene Name : ITK IL2-inducible T-cell kinase [ Homo sapiens ]
Official Symbol : ITK
Synonyms : ITK; IL2-inducible T-cell kinase; tyrosine-protein kinase ITK/TSK; EMT; LYK; PSCTK2; kinase EMT; T-cell-specific kinase; tyrosine-protein kinase LYK; IL-2-inducible T cell kinase; IL-2-inducible T-cell kinase; homolog of mouse T-cell itk/tsk; interleukin-2-inducible T cell kinase; interleukin-2-inducible T-cell kinase; MGC126257; MGC126258;
Gene ID : 3702
mRNA Refseq : NM_005546
Protein Refseq : NP_005537
MIM : 186973
UniProt ID : Q08881

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (5)

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What is the significance of ITK in allergic and autoimmune diseases, and how does it contribute to immune dysregulation in these conditions? 01/10/2022

ITK's involvement in Th2 cell differentiation links it to allergic diseases. Dysregulation of ITK is implicated in autoimmune diseases, influencing aberrant T-cell responses and immune system dysfunction.

Can you discuss the role of ITK in the context of immunodeficiency disorders, and how genetic variations impact its function? 12/01/2021

Deficiencies in ITK function are associated with immunodeficiency disorders. Genetic variations in ITK may compromise its kinase activity, leading to impaired T-cell signaling and immune responses.

What is the primary role of the ITK protein in T-cell signaling, and how does it contribute to immune responses? 10/17/2020

ITK is a tyrosine kinase involved in T-cell signaling, specifically in the activation of T-cell receptors. It plays a critical role in the regulation of immune responses and T-cell-mediated immunity.

Can you discuss the molecular mechanisms by which ITK is activated and its downstream signaling pathways in T cells? 02/27/2019

ITK is activated by phosphorylation and participates in downstream signaling pathways, including the PLCγ1 pathway, leading to the mobilization of calcium ions and activation of NFAT transcription factors.

How does ITK participate in the regulation of immune synapse formation, and what are the implications for T-cell activation and effector functions? 09/03/2018

ITK contributes to immune synapse formation by mediating cytoskeletal changes and promoting T-cell receptor clustering. This influences T-cell activation and the subsequent effector functions.

Customer Reviews (3)

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Reviews
06/28/2021

    Research-grade excellence. This product has simplified our experiments, and the quality is unmatched.

    06/15/2020

      The shipping speed surprised me! Got what I needed for our experiments right on schedule.

      10/06/2018

        Customer service that goes above and beyond. They truly understand our lab's needs

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