Recombinant Human SPP1, His-tagged SPP1-616H

Recombinant Human SPP1, His-tagged

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Recombinant Human SPP1, His-tagged

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Cat.No. : SPP1-616H
Product Overview : Recombinant Human SPP1 (Accession # NP_000573.1) Ile17-Asn300, fused with a C-terminal 6-His tag, was produced in Mouse myeloma cell line, NS0-derived.
Source : NSO cells
Species : Human
Tag : His
Predicted N Terminal : Ile17
Form : Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
Bio-activity : Measured by the ability of the immobilized protein to support the adhesion of HEK293 human embryonic kidney cells. Agnihotri, R. et al. (2001) J. Biol. Chem. 276:28261.Recombinant Human (rh) Osteopontin/OPN immobilized at 1 µg/mL, 100 µL/well, will mediate the adhesion of added 293 cells >30% (1 x 10^5 cells/well). Recombinant Human Coagulation Factor II/Thrombin proteolytic treatment of this rhOsteopontin/his can increase HEK293 cell adhesion by about 5-fold.
Molecular Mass : Recombinant Human SPP1, His-tagged has a calculated MW of 32.9 kDa. In SDS-PAGE migrates as 60-65 kDa, reducing conditions.
Purity : >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage : Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Reconstitution : Reconstitute at 100 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Gene Name : PPP3CA protein phosphatase 3, catalytic subunit, alpha isozyme [ Homo sapiens ]
Official Symbol : PPP3CA
Synonyms : PPP3CA; protein phosphatase 3, catalytic subunit, alpha isozyme; CALN, CALNA, protein phosphatase 3 (formerly 2B), catalytic subunit, alpha isoform , protein phosphatase 3 (formerly 2B), catalytic subunit, alpha isoform (calcineurin A alpha); serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform; calcineurin A alpha; CNA1; PPP2B; protein phosphatase 2B; catalytic subunit; alpha isoform; CAM-PRP catalytic subunit; calmodulin-dependent calcineurin A subunit alpha isoform; protein phosphatase 2B, catalytic subunit, alpha isoform; protein phosphatase 3 (formerly 2B), catalytic subunit, alpha isoform; CALN; CCN1; CALNA; CALNA1;
Gene ID : 5530
mRNA Refseq : NM_000944
Protein Refseq : NP_000935
MIM : 114105
UniProt ID : Q08209
Chromosome Location : 4q24

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