Active Recombinant Human SPP1, His-tagged
Cat.No. : | SPP1-616H |
Product Overview : | Recombinant Human SPP1 (Accession # NP_000573.1) Ile17-Asn300, fused with a C-terminal 6-His tag, was produced in Mouse myeloma cell line, NS0-derived. |
- Specification
- Gene Information
- Related Products
Source : | Mammalian cells |
Species : | Human |
Tag : | His |
Predicted N Terminal : | Ile17 |
Form : | Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein. |
Bio-activity : | Measured by the ability of the immobilized protein to support the adhesion of HEK293 human embryonic kidney cells. Agnihotri, R. et al. (2001) J. Biol. Chem. 276:28261.Recombinant Human (rh) Osteopontin/OPN immobilized at 1 µg/mL, 100 µL/well, will mediate the adhesion of added 293 cells >30% (1 x 10^5 cells/well). Recombinant Human Coagulation Factor II/Thrombin proteolytic treatment of this rhOsteopontin/his can increase HEK293 cell adhesion by about 5-fold. |
Molecular Mass : | Recombinant Human SPP1, His-tagged has a calculated MW of 32.9 kDa. In SDS-PAGE migrates as 60-65 kDa, reducing conditions. |
Purity : | >95%, by SDS-PAGE under reducing conditions and visualized by silver stain. |
Storage : | Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC). |
Reconstitution : | Reconstitute at 100 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
Gene Name : | PPP3CA protein phosphatase 3, catalytic subunit, alpha isozyme [ Homo sapiens ] |
Official Symbol : | PPP3CA |
Synonyms : | PPP3CA; protein phosphatase 3, catalytic subunit, alpha isozyme; CALN, CALNA, protein phosphatase 3 (formerly 2B), catalytic subunit, alpha isoform , protein phosphatase 3 (formerly 2B), catalytic subunit, alpha isoform (calcineurin A alpha); serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform; calcineurin A alpha; CNA1; PPP2B; protein phosphatase 2B; catalytic subunit; alpha isoform; CAM-PRP catalytic subunit; calmodulin-dependent calcineurin A subunit alpha isoform; protein phosphatase 2B, catalytic subunit, alpha isoform; protein phosphatase 3 (formerly 2B), catalytic subunit, alpha isoform; CALN; CCN1; CALNA; CALNA1; |
Gene ID : | 5530 |
mRNA Refseq : | NM_000944 |
Protein Refseq : | NP_000935 |
MIM : | 114105 |
UniProt ID : | Q08209 |
Chromosome Location : | 4q24 |
Products Types
◆ Recombinant Protein | ||
Spp1-2095M | Recombinant Mouse Spp1 Protein, His-tagged | +Inquiry |
SPP1-5246H | Recombinant Human SPP1 Protein, GST-tagged | +Inquiry |
SPP1-1164P | Recombinant Pig SPP1 Protein, His-tagged | +Inquiry |
SPP1-001H | Recombinant Human SPP1 Protein, MBP-tagged | +Inquiry |
SPP1-5378R | Recombinant Rat SPP1 Protein, His (Fc)-Avi-tagged | +Inquiry |
◆ Lysates | ||
SPP1-2535HCL | Recombinant Human SPP1 cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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