Active Recombinant Human ITGAM&ITGB2

• Cat.No.: ITGAM&ITGB2-692H
• Product Overview: Recombinant Human ITGAM&ITGB2 (Accession # NP_001139280 (Integrin alpha M) & P05107 (Integrin beta 2)) was produced in Chinese Hamster Ovary cell line, CHO-derived.

Specification

• Species: Human
• Source: CHO
• Tag: Non
• Predicted N Terminal: Phe17 (Integrin alpha M) & No results obtained: Gln23 predicted (Integrin beta 2)
• Form: Lyophilized from a 0.2 µm filtered solution in Tris, NaCl and MgCl2.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of CHO Chinese hamster ovary cells transfected with ICAM-1.The ED50 for this effect is typically 0.25-1.5 µg/mL.
• Molecular Mass: Recombinant Human ITGAM&ITGB2 has a calculated MW of 124.7 kDa (Integrin alpha M), 79 kDa (Integrin beta 2). In SDS-PAGE migrates as 165 kDa and 100 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: ITGAM integrin, alpha M (complement component 3 receptor 3 subunit) [ Homo sapiens ]
• Official Symbol: ITGAM
• Synonyms: ITGAM; integrin, alpha M (complement component 3 receptor 3 subunit); CD11B, CR3A, integrin, alpha M (complement component receptor 3, alpha; also known as CD11b (p170), macrophage antigen alpha polypeptide); integrin alpha-M; CD11b; MAC 1; CR-3 alpha chain; antigen CD11b (p170); leukocyte adhesion receptor MO1; CD11 antigen-like family member B; macrophage antigen alpha polypeptide; cell surface glycoprotein MAC-1 subunit alpha; neutrophil adherence receptor alpha-M subunit; CR3A; MO1A; CD11B; MAC-1; MAC1A; SLEB6; MGC117044
• Gene ID: 3684
• mRNA Refseq: NM_000632
• Protein Refseq: NP_000623
• MIM: 120980
• UniProt ID: P11215
• Chromosome Location: 16p11.2