||Recombinant Human ITGAM&ITGB2 (Accession # NP_001139280 (Integrin alpha M) & P05107 (Integrin beta 2)) was produced in Chinese Hamster Ovary cell line, CHO-derived.
|Predicted N Terminal:
||Phe17 (Integrin alpha M) & No results obtained: Gln23 predicted (Integrin beta 2)
||Lyophilized from a 0.2 µm filtered solution in Tris, NaCl and MgCl2.
||Measured by the ability of the immobilized protein to support the adhesion of CHO Chinese hamster ovary cells transfected with ICAM-1.The ED50 for this effect is typically 0.25-1.5 µg/mL.
||Recombinant Human ITGAM&ITGB2 has a calculated MW of 124.7 kDa (Integrin alpha M), 79 kDa (Integrin beta 2). In SDS-PAGE migrates as 165 kDa and 100 kDa, reducing conditions.
||>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
||Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).