Active Recombinant Human GCNT1, His-tagged

• Cat.No.: GCNT1-714H
• Product Overview: Recombinant Human GCNT1 (Accession # NP_001481) Arg33-His428, fused with an N-terminal CD33 signal sequence and a C-terminal 6-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Human
• Tag: His
• Predicted N Terminal: Arg33
• Form: Supplied as a 0.2 µm filtered solution in Tris, NaCl and Brij-35.
• Bio-activity: Measured by its ability to transfer GlcNAc from UDP-GlcNAc to B1-3 galactosyl-N-acetyl galactosamine.The specific activity is >350 pmol/min/µg, as measured under the described conditions.
• Molecular Mass: Recombinant Human GCNT1, His-tagged has a calculated MW of 47 kDa. In SDS-PAGE migrates as 50-57 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 µg per lane.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: ITGAV integrin, alpha V [ Homo sapiens ]
• Official Symbol: ITGAV
• Synonyms: ITGAV; integrin, alpha V; antigen identified by monoclonal L230 , integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51) , MSK8, vitronectin receptor , VNRA, VTNR; integrin alpha-V; CD51; integrin alphaVbeta3; vitronectin receptor subunit alpha; antigen identified by monoclonal L230; integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51); MSK8; VNRA; VTNR; DKFZp686A08142
• Gene ID: 3685
• mRNA Refseq: NM_001144999
• Protein Refseq: NP_001138471
• MIM: 193210
• UniProt ID: P06756
• Chromosome Location: 2q31-q32